GRP78 BiP Mouse mAb - Primary antibody, specific to GRP78 BiP, Mouse IgG2b

Cat. No.: Ab106528

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, WB

Host species

Mouse

Reactive species

Human, Mouse

Isotype

Mouse IgG2b

Clone Number

C38

Conjugation

Unconjugated

Descripción general

Mouse anti Human/Mouse GRP78 BiP Antibody, Monoclonal (C38), could be used for in vivo GRP78 blockade, in vitro GRP78 blockade, WB, Flow and so on.

Application:

in vivo GRP78 blockade: Use at an assay dependent concentration. 

in vitro GRP78 blockade: Use at an assay dependent concentration. 

WB: Use at an assay dependent concentration.

Flow: Use at an assay dependent concentration.
Protein Function
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Specifications

Product Name

GRP78 BiP Mouse mAb - Primary antibody, specific to GRP78 BiP, Mouse IgG2b

Sinónimos

HEL-S-89n | Bip | Sez7 | mBiP | Grp78 | SEZ-7 | Hsce70 | baffled | D2Wsu17e | D2Wsu141e | 78 kDa glucose-regulated protein | BIP | Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 | FLJ26106 | GRP78 | GRP-78 | GRP78BIP | Heat shock 70 kDa protei

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Especies hospedadoras

Mouse

Especificidad

GRP78 BiP

Inmunógeno

Recombinant protein of murine GRP78 (AA 1-655)

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotipo

Mouse IgG2b

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Imágenes

Flow Cytometry: GRP78 BiP Mouse mAb (Ab106528)

GRP78 BiP Mouse mAb (Ab106528) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling GRP78 BiP (red) with GRP78 BiP Mouse mAb (Ab106528) at 0.1 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• WB:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo GRP78 blockade:

  Use at an assay dependent concentration ;

• in vitro GRP78 blockade:

  Use at an assay dependent concentration ;

Objetivos asociados (humanos)

HSPA5 Tchem Endoplasmic reticulum chaperone BiP (0 Activities)

Discover Target: HSPA5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción