H-2Kb bound to SIINFEKL Mouse mAb (Biotin) - Primary antibody, specific to H-2Kb bound to SIINFEKL

Cat. No.: Ab209992

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG1

Clone Number

25-D1.16

Conjugation

Biotin

Descripción general

Mouse anti Mouse H-2Kb bound to SIINFEKL Antibody, Monoclonal (25-D1.16), could be used for Flow.

Specifications

Product Name

H-2Kb bound to SIINFEKL Mouse mAb (Biotin) - Primary antibody, specific to H-2Kb bound to SIINFEKL

Sinónimos

A 28 antibody | A 9 antibody | Antigen presenting molecule antibody | Aw 24 antibody | Aw 68 antibody | CLASS I HISTOCOMPATIBILITY ANTIGEN antibody | H2 K1 antibody | H2K antibody | HLA A antibody | HLA class I histocompatibility antigen A 1 alpha chain a

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Especies hospedadoras

Mouse

Especificidad

H-2Kb bound to SIINFEKL

Inmunógeno

SIINFEKL pulsed RMA-S cells

Control positivo

Flow: C57BL/6 mouse splenocytes​

Conjugación

Biotin

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: H-2Kb bound to SIINFEKL Mouse mAb (Biotin) (Ab209992)

H-2Kb bound to SIINFEKL Mouse mAb (Biotin) (Ab209992) - Flow Cytometry
C57BL/6 mouse splenocytes cells treated with 30μM OVA Peptide for 2 hours (right panel) were stained with H-2Kb bound to SIINFEKL Mouse mAb (Biotin) (Ab209992) (red) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody. Untreated spleen cells were used as control (left panel).

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test. For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

HLA-A Tbio HLA class I histocompatibility antigen, A-69 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)