IGFBP3 Mouse mAb - Primary antibody, specific to IGFBP3, Mouse IgG1

Cat. No.: Ab109567

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, See COA

Application

WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

D15

Conjugation

Unconjugated

Descripción general

Mouse anti Human IGFBP3 Antibody, Monoclonal (D15), could be used for WB and so on.

Application:

WB: 0.5-2µg/mL

Protein Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Insulin like growth factor binding protein 3 (IGFBP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGFBP3 is fused to the signal peptide of CD33. Human IGFBP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. Insulin like growth factor binding protein 3 (IGFBP3) can modulate the mitogenic and metabolic effects of the insulin like growth factors (IGFs). Insulin like growth factor binding protein 3 (IGFBP3) is expressed in multiple tissues. The highest expression level is found in the non paranchymal cells of the liver. The expression levels are higher during extrauterine life and peak during puberty. Insulin like growth factors (IGFs) and IGF binding proteins (IGFBPs) play important roles in cell growth and differentiation. IGFBP3 is one of the factors in serum that is responsible for high serum induced apoptosis in PC3 cells, a prostate cancer cell line. IGFBP3 is important in controlling glucose homeostasis with increased urinary levels in type I diabetes with persistent microalbuminuria.

Specifications

Product Name

IGFBP3 Mouse mAb - Primary antibody, specific to IGFBP3, Mouse IgG1

Sinónimos

Acid stable subunit of the 140 K IGF complex antibody | Binding protein 29 antibody | Binding protein 53 antibody | BP 53 antibody | BP53 antibody | Growth hormone dependent binding proteinantibody | IBP 3 antibody | IBP3 antibody | IGF binding protein 3

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, See COA

Especies hospedadoras

Mouse

Especificidad

IGFBP3

Inmunógeno

Recombinant IGFBP3 expressed in E.coli (AA 135-277)

Control positivo

WB: A431 and Hep G2 cell lysates.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A + Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: IGFBP3 Mouse mAb (Ab109567)

IGFBP3 Mouse mAb (Ab109567) - Western Blot
All lanes: IGFBP3 Mouse mAb (Ab109567) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 37 kDa
Exposure time: 9.1 s

Aplicación

• Aplicación: Dilution info
• WB:

  0.5-2µg/mL;

Objetivos asociados (humanos)

IGFBP3 Tchem Insulin-like growth factor-binding protein 3 (0 Activities)

Discover Target: IGFBP3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción