IL-10 Mouse mAb - Primary antibody, specific to IL10, Mouse IgG1

Cat. No.: Ab109816

CALIDAD & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, See COA

Application

Capture Antibody, CLIA, ELISA, FIA, FLFIA, GICA, LF

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

1B4-15

Conjugation

Unconjugated

Descripción general

Mouse anti Human IL-10 Antibody, Monoclonal (1B4-15), is a Capture antibody could be used for ELISA, CLIA, LF, GICA, FIA, FACS.
CLIA: 20µg/2mg magnetic beads
FLFIA: Fluorescent beads/Antibody = 10/1

Especificaciones

Product Name

IL-10 Mouse mAb - Primary antibody, specific to IL10, Mouse IgG1

Sinónimos

CSIF | CSIFMGC126450 | Cytokine synthesis inhibitory factor | GVHDS | IL10 | IL-10 | IL10A | IL-10MGC126451 | interleukin 10 | interleukin-10 | TGIF | CSIF antibody | Cytokine synthesis inhibitory factor antibody | GVHDS antibody | IL 10 antibody | IL-10

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA

Especies hospedadoras

Mouse

Especificidad

IL10

Inmunógeno

Recombinant Human IL-10 Protein

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

25.5 kDa (Light Chain) & 50.0 kDa (Heavy Chain), under reducing conditions; 172.4 kDa, under non-reducing conditions.

Método de purificación

Affinity chromatography

Pares emparejados

[Ab109817] IL-10 Mouse mAb as Detection antibody in ELISA, CLIA, LF, GICA, FIA, FACS

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 37°C short term（7 days）Store at 4°C short term（30 days）. Store at -20±5°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Sandwich ELISA - IL-10 Mouse mAb Pair (Ab109816 & Ab109817)

Sandwich ELISA - IL-10 protein standard curve
Human IL-10 protein was serially diluted 2-fold and captured by IL-10 Mouse mAb (Ab109816) coated on a Clear Polystyrene Microplate (W291037). IL-10 Mouse mAb (Ab109817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).

Relevance of detection results between aladdin Antibody Pair (Ab109816 & Ab109817) in CLIA

Relevance of detection results between aladdin Antibody Pair (Ab109816 & Ab109817) in CLIA
·Capture antibody: IL-10 Mouse mAb (Ab109816)
·Detection antibody: IL-10 Mouse mAb (Ab109817)

Binding ELISA - IL-10 Mouse mAb (Ab109816)

Binding ELISA - IL-10 Mouse mAb (Ab109816)
Immobilized Recombinant Human IL-10 Protein at 1.0 μg/mL can bind IL-10 Mouse mAb (Ab109816) with the EC₅₀ of 45.78 ng/mL.

Aplicación

Aplicación

Dilution info

CLIA

20µg/2mg magnetic beads

FLFIA

Fluorescent beads/Antibody =10/1

Objetivos asociados (humanos)

IL10 Tbio Interleukin-10 (0 Activities)

Discover Target: IL10

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IL-10 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.