Lamin A/C Mouse mAb - Primary antibody, specific to Lamin A

Cat. No.: Ab210151

GRADE & PURITY Animal Free, Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Human, Rat

Isotype

Mouse IgG2b

Clone Number

AB07/4G6

Conjugation

Unconjugated

Descripción general

Mouse anti Human Lamin A/C Antibody, clone AB07/4G6, could be used for WB, Flow cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name

Lamin A/C Mouse mAb - Primary antibody, specific to Lamin A

Sinónimos

70 kDa lamin antibody | Cardiomyopathy dilated 1A (autosomal dominant) antibody | CDCD1 antibody | CDDC antibody | CMD1A antibody | CMT2B1 antibody | EMD2 antibody | FPL antibody | FPLD antibody | FPLD2 antibody | HGPS antibody | IDC antibody | Lamin A an

Especificaciones y pureza

Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal

Especies hospedadoras

Mouse

Especificidad

Lamin A

Inmunógeno

Recombinant protein corresponding to Human Lamin A

Control positivo

WB: HeLa, A549, MCF7, THP-1, K562 and C6 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells

Conjugación

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Western Blot: Lamin A/C Mouse mAb (Ab210151)

Lamin A/C Mouse mAb (Ab210151) - Western Blot
All lanes: Lamin A/C Mouse Antibody (Ab210151) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 74, 65 kDa
Observed band size: 72, 65 kDa
Exposure time: 10.0 s

Flow Cytometry: Lamin A/C Mouse mAb (Ab210151)

Lamin A/C Mouse mAb (Ab210151) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Lamin A/C Mouse mAb (Ab210151) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

ICC/IF: Lamin A/C Mouse mAb (Ab210151)

Lamin A/C Mouse mAb (Ab210151) - ICC/IF
IF analysis of Lamin A/C (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Lamin A/C Mouse mAb (Ab210151) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1/200;

• WB:

  1/20000 – 1/200000;

• Flow Cytometry:

  1/800 – 1/50000;

Objetivos asociados (humanos)

LMNA Tbio Prelamin-A/C (0 Activities)

Discover Target: LMNA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción