GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Human Lamin B1 Antibody, Monoclonal (C2), could be used for WB, IHC and so on. Application: WB: 0.1ng/ml-100ng/mL IHC: 5-20μg/mL Protein Function Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Post-translational B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Specifications
Product Name
Lamin B1 Mouse mAb - Primary antibody, specific to LMNB1, Mouse IgG2b
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Especies hospedadoras
Mouse
Especificidad
LMNB1
Inmunógeno
Recombinant LMNB1 expressed in E.coli (AA 52-233).
Control positivo
WB: MCF7, HeLa and Raw264.7 cell lysates. IHC: Human Kidney Tissue, Human Stomach Tissue, Human Ovarian cancer Tissue, Human Thyroid cancer Tissue, Human amy.gdalitis Tissue and Human Lung cancer Tissue.
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Mouse IgG2b
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A+G Affinity Chromatography
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Lamin B1 Mouse mAb (Ab112658) - Western Blot All lanes: Lamin B1 Mouse mAb (Ab112658) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 66 kDa Observed band size: 68 kDa Exposure time: 30 s
Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mLHRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody .
Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Stomach Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Ovarian cancer Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Thyroid cancer Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human amy.gdalitis Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Lamin B1 Mouse mAb (Ab112658) - IHC DAB staining on IHC Samples: Human Lung cancer Tissue Primary Ab: 10µg/mL Lamin B1 Mouse mAb (Ab112658) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
Aplicación
Aplicación
Dilution info
IHC
5-20 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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