MAGEA1 Antibody - Primary antibody, specific to MAGEA1, Rabbit IgG

Cat. No.: Ab114011

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Monkey

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human MAGEA1 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/1000
IHC: 1/100 - 1/200

IF/ICC: 1/200
Protein Function
May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Specifications

Product Name

MAGEA1 Antibody - Primary antibody, specific to MAGEA1, Rabbit IgG

Sinónimos

Antigen MZ2 E antibody | Antigen MZ2-E antibody | Cancer/testis antigen 1.1 antibody | cancer/testis antigen family 1, member 1 antibody | CT1.1 antibody | MAGA1_HUMAN antibody | MAGE 1 antigen antibody | MAGE-1 antigen antibody | MAGE1 antibody | MAGE1A

Especificaciones y pureza

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Especies hospedadoras

Rabbit

Especificidad

MAGEA1

Inmunógeno

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MAGEA1.

Control positivo

WB: UWB: U-2 OS, A375 and A431 cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: MAGEA1 Antibody (Ab114011)

MAGEA1 Antibody (Ab114011) - Western Blot
All lanes: MAGEA1 Antibody (Ab114011) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 44 kDa
Exposure time: 0.3 s

IHC: MAGEA1 Antibody (Ab114011)

MAGEA1 Antibody (Ab114011) - IHC
Immunohistochemical analysis of MAGEA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with MAGEA1 Antibody (Ab114011) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: MAGEA1 Antibody (Ab114011)

MAGEA1 Antibody (Ab114011) - IF/ICC
IF analysis of MAGEA1 Antibody (Ab114011) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MAGEA1 Antibody (Ab114011) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Aplicación

• Aplicación: Dilution info
• IHC:

  1/100 - 1/200;

• IF/ICC:

  1/200;

• WB:

  1/500-1/1000 ;

Objetivos asociados (humanos)

MAGEA1 Tbio Melanoma-associated antigen 1 (0 Activities)

Discover Target: MAGEA1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)