MDX-1105 (anti-PD-L1) - Primary antibody, specific to CD274, Human IgG4SP

Cat. No.: Ab190151

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Human

Reactive species

Cynomolgus monkey, Human

Isotype

Human IgG1

Conjugation

Unconjugated

Descripción general

MDX-1105 (anti-PD-L1) is a fully humanized monoclonal antibody targeting B7-H1. It is used in research of advanced melanoma, non-small cell lung carcinoma, renal cell carcinoma, and ovarian cancer.

Specifications

Product Name

MDX-1105 (anti-PD-L1) - Primary antibody, specific to CD274, Human IgG4SP

Sinónimos

B7 H antibody | B7 H1 antibody | B7 homolog 1 antibody | B7-H1 antibody | B7H antibody | B7H1 antibody | CD 274 antibody | CD-274 antibody | CD274 antibody | CD274 antigen antibody | CD274 molecule antibody | MGC142294 antibody | MGC142296 antibody | OTTH

Especificaciones y pureza

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC), See COA

Mecanismos bioquímicos y fisiológicos

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Especies hospedadoras

Human

Especificidad

CD274

Conjugación

Unconjugated

Grado

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Isotipo

Human IgG4SP

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -80°C,Avoid repeated freezing and thawing

Enviado en

Dry ice packs + Cold packs

Estabilidad y almacenamiento

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Flow Cytometry: MDX-1105 (anti-PD-L1) (Ab190151)

MDX-1105 (anti-PD-L1) (Ab190151) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated (right) Jurkat cells labelling PD-L1 (red) with MDX-1105 (anti-PD-L1) (Ab190151) at 0.1 μg/mL for 1 hour at 4°C. Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

ELISA: MDX-1105 (anti-PD-L1) (Ab190151)

MDX-1105 (anti-PD-L1) (Ab190151) - ELISA
Immobilized Recombinant Human PD-L1 protein (rp169666) at 1.0 μg/mL can bind MDX-1105 (anti-PD-L1) (Ab190151) with the EC50 of 158.5 ng/mL.

SEC: MDX-1105 (anti-PD-L1) (Ab190151)

MDX-1105 (anti-PD-L1) (Ab190151) - SEC
The purity of MDX-1105 (anti-PD-L1) (Ab190151) is more than 95% verified by HPLC.

Objetivos asociados (humanos)

CD274 Tclin Programmed cell death 1 ligand 1 (0 Activities)

Discover Target: CD274

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción