GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Validated, ExactAb™, 1.0 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human MEK5 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/1000-1/2000 IHC: 1/200-1/500 Protein Function： Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
Specifications
Product Name
MEK5 Antibody - Primary antibody, specific to MAP2K5, Rabbit IgG
WB: HEK293, HeLa and NIH/3T3 cell lysates. IHC: Human breast carcinoma tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Immunogen affinity purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
MEK5 Antibody (Ab114893) - Western Blot All lanes: MEK5 Antibody (Ab114893) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa Observed band size: 49 kDa Exposure time: 100.0 s
MEK5 Antibody (Ab114893) - IHC Immunohistochemistry analysis of paraffin-embedded Human Breast Carcinoma tissue using MEK5 Antibody (Ab114893). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
MEK5 Antibody (Ab114893) - IF/ICC IF analysis of MEK5 Antibody (Ab114893) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MEK5 Antibody (Ab114893) at 1/300 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
WB
1/1000-1/2000
IHC
1/200-1/500
IF/ICC
1/300
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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