NME4 Mouse mAb - Primary antibody, specific to NME4

Cat. No.: Ab118326

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

OTI5E4

Conjugation

Unconjugated

Descripción general

Rabbit anti Human NME4 Antibody, Monoclonal (OTI5E4), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

NME4 Mouse mAb - Primary antibody, specific to NME4

Sinónimos

NME4 | NDPK-D | NM23H4 | nm23-H4 | metastatic inhibition factor NM23H4 antibody | mitochondrial antibody | NDK antibody | NDKM_HUMAN antibody | NDP kinase antibody | NDP kinase D antibody | NDP kinase | NDPK D antibody | NDPKD antibody | nm23 H4 antibody

Especificaciones y pureza

ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

Major role in the synthesis of nucleoside triphosphates other than ATP.

Especies hospedadoras

Mouse

Especificidad

NME4

Inmunógeno

Recombinant Full length protein of human NME4.

Control positivo

WB: Hep G2, A549 and Jurkat cell lysates. IHC: Human colon, kidney, liver, ovary tissue. IF/ICC: COS7 cells transiently transfected by pCMV6-ENTRY NME4. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY NME4.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - Western Blot
All lanes: NME4 Mouse mAb (Ab118326) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 17 kDa
Exposure time: 71.5 s

Flow Cytometry: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY NME4(Red) or empty vector control plasmid (Blue) were immunostained with NME4 Mouse mAb (Ab118326), and then analyzed by flow cytometry.

IF/ICC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IF/ICC
NME4 Mouse mAb (Ab118326) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NME4.

IHC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of colon tissue using NME4 Mouse mAb (Ab118326). (Heat-induced epitope retrieval by 10-mM citric buffer, pH-6.0, 100°C for 10 min, Dilution 1/50).

IHC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IHC
Immunohistochemical staining of paraffinembedded Kidney tissue within the normal limits using NME4 Mouse mAb (Ab118326). (Heat-induced epitope retrieval by 10-mM citric buffer, pH-6.0, 100°C for 10 min, Dilution 1/50).

IHC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IHC
Immunohistochemical staining of paraffinembedded liver tissue within the normal limits using NME4 Mouse mAb (Ab118326). (Heat-induced epitope retrieval by 10-mM citric buffer, pH-6.0, 100°C for 10 min, Dilution 1/50).

IHC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of ovary tissue using NME4 Mouse mAb (Ab118326). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

Aplicación

• Aplicación: Dilution info
• WB:

  1/2000 ;

• IF/ICC:

  1/100;

• Flow Cytometry:

  1/100;

• IHC:

  1/50

Objetivos asociados (humanos)

NME4 Tbio Nucleoside diphosphate kinase, mitochondrial (0 Activities)

Discover Target: NME4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)