CALIDAD & PUREZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.See COA
Carrier Free, ExactAb™, Validated, See COA Carrier Free,ExactAb™,Validated para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.
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Almacenamiento y envío
Store at -20°C,Avoid repeated freezing and thawing Envío Ice chest + Ice pads. Consulte el COA específico del lote para conocer las especificaciones exactas.
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Documentos de calidad
SDS, COA, ficha técnica y hoja de especificaciones disponibles para descargar. COA específico del lote accesible mediante búsqueda por número de lote.
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Prueba documental
Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.
Descripción general
Mouse anti Human NSE Antibody, Monoclonal (C10), could be used for WB, IHC, ICC, IF and so on. Application: WB: 0.5-2µg/mL IHC: 5-30µg/mL ICC/IF: 5-30µg/mL Protein Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Especificaciones
Product Name
NSE Mouse mAb - Primary antibody, specific to NSE, Mouse IgG2b
WB: Hep G2, NIH/3T3, C6 and Jurkat cell lysates. IHC: Human Glioma Tissue, Human Cerebrum Tissue. IF/ICC: Hep G2 cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Mouse IgG2b
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A + Protein G purified
Concentración
See COA
Product Line
Productos biológicos
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
NSE Mouse mAb (Ab118776) - Western Blot All lanes: NSE Mouse mAb (Ab118776) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 16.4 s
NSE Mouse mAb (Ab118776) - IHC DAB staining on IHC Samples: Human Glioma Tissue Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
NSE Mouse mAb (Ab118776) - IHC DAB staining on IHC Samples: Human Cerebrum Tissue Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
NSE Mouse mAb (Ab118776) - IF/ICC FITC staining on IF Samples: Human Hep G2 cells Primary Ab: 20µg/mL NSE Mouse mAb (Ab118776) Second Ab: 1µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Aplicación
Aplicación
Dilution info
IHC
5-30 µg/mL
WB
0.5-2 µg/mL
IF/ICC
5-30 µg/mL
Documentación
📋 Hoja de datos de seguridad (SDS)
Documento completo sobre peligros, manipulación, almacenamiento y cumplimiento normativo.
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Reseñas
Reseñas de cliente
Preguntas frecuentes
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (NSE Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
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