Nuclease micrococcal from Staphylococcus aureus(Strain ATCC #27735), CAS No.9013-53-0

CAS: 9013-53-0 Cat. No.: N128635 Número EC: 232-748-0

GRADE & PURITY EnzymoPure™, ≥6,000 units/mg protein

Descripción general

Characteristics of Micrococcal Nuclease from S. aureus:

Specificity

Exhibits both exo- and endo-5'-phosphodiesterase activities against both DNA and RNA (Alexander et al. 1961). The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate (Roberts et al. 1962; Rushisky et al. 1962).

Composition

The amino acid sequence has been reported by Cone et al. (1971). The active site has been reported on by Chaiken and Anfinsen (1971), Cuatrecasas (1970), and Cuatrecasas et al. (1968). See also: Furie et al. 1973), Bonhert and Taniuchi (1972), Sacks et al. (1972), Arnone et al. (1971), Epstein et al. (1971), Chaiken and Anfinsen (1970), Cohen et al. (1970), Markley et al. (1970), Putter et al. (1970), and Schechter et al. (1970).

Characteristics of Nuclease, Micrococcal

Molecular Weight

16,807 (Taniuchi et al. 1967b).

Optimal pH

The pH optimum is reported as 9.2. However, the enzymic activity has an absolute requirement for Ca2+ and the pH optimum varies according to Ca2+ concentration (Heins et al. 1967).

Extinction Coefficient

 = 9.2 (Fuchs et al. 1967).

Inhibitors

5'-dioxynucleotides and 5'-ribonucleotides are inhibitory with the former demonstrating greatest inhibition.

Activity

Ca2+ is essential for activity. (See, however, Frank et al. 1975). Both RNase and DNase activities are competitively inhibited by deoxythymidine 3',5' diphosphate (Cuatrecasas et al. 1967). Sulkowski and Laskowski, Sr. (1970) indicate that the kinetics of degradation of RNA and DNA differ. They found no activation with Sr2+. See also: Dunn et al. (1973), Rammler et al. (1972), Chaiken and Anfinsen (1971), Markley and Jardetzy (1970) and Mikulski et al. (1969).

Specifications

Product Name

Nuclease micrococcal from Staphylococcus aureus(Strain ATCC #27735), CAS No.9013-53-0

Grado

EnzymoPure™

Especificaciones y pureza

EnzymoPure™, ≥6, 000 units/mg protein

Mecanismos bioquímicos y fisiológicos

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C. Hydrolyzes 5′-phosphodiester bonds of DNA.

CAS

9013-53-0

Número de la Comisión de Enzimas

3.1.31.1

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

≥6,000 units/mg protein

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Definición de unidad

Unit Definition: One Unit corresponds to a change in optical density of 1.0 at 260 nm at 37°C, pH 8.0, using DNA as the substrate.

Referencias

1. Zhang Liangliang, Zhu Dagang, Jiang Jiwen, Min Zhenyu, Fa Zhenzhong.  (2023)  The ubiquitin E3 ligase MDM2 induces chemoresistance in colorectal cancer by degradation of ING3.  CARCINOGENESIS,      [PMID:37279970] [10.1093/carcin/bgad040]