PDPK1 Mouse mAb - Primary antibody, specific to PDPK1, IgG1

Cat. No.: Ab121091

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

3H3D9

Conjugation

Unconjugated

Descripción general

Application:
WB: 1/500 - 1/2000. Predicted molecular weight: 63 kDa.
IF/ICC: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000

Protein Function
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.

Specifications

Product Name

PDPK1 Mouse mAb - Primary antibody, specific to PDPK1, IgG1

Sinónimos

EC 2.7.11.1 | hPDK1

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0mg/mL

Especies hospedadoras

Mouse

Especificidad

PDPK1

Inmunógeno

Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.

Control positivo

WB: C6, K562, NIH/3T3 and MCF7 cell lysates. Flow: HeLa cells. ICC/IF: HeLa cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

1.0mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Imágenes

Western Blot: PDPK1 Mouse mAb (Ab121091)

PDPK1 Mouse mAb (Ab121091) - Western Blot
All lanes: PDPK1 Mouse mAb (Ab121091) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 64 kDa
Observed band size: 51- 64 kDa
Exposure time: 113.7 s

ICC/IF: PDPK1 Mouse mAb (Ab121091)

PDPK1 Mouse mAb (Ab121091) - ICC/IF
Immunofluorescence analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AF555 phalloidin.

Flow Cytometry: PDPK1 Mouse mAb (Ab121091)

PDPK1 Mouse mAb (Ab121091) - Flow Cytometry
Flow cytometric analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green) and negative control (red).

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1/200-1/1000 ;

• Flow Cytometry:

  1/200-1/400 ;

• ELISA:

  1/10000 ;

Objetivos asociados (humanos)

PDPK1 Tchem 3-phosphoinositide-dependent protein kinase 1 (0 Activities)

Discover Target: PDPK1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción