Anticuerpo Pentraxina 3/PTX3 - Primary antibody, specific to Pentraxin 3/PTX3, Rabbit IgG

Cat. No.: Ab121192

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Cow, Human, Mouse, Pig, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Anticuerpo de conejo anti Pentraxina 3/PTX3 humana, policlonal, puede utilizarse para WB, IHC, ICC, IF, etc.
Aplicación:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Función de la proteína
Interviene en la regulación de la resistencia innata a patógenos, las reacciones inflamatorias, posiblemente la eliminación de autocomponentes y la fertilidad femenina

Specifications

Product Name

Anticuerpo Pentraxina 3/PTX3 - Primary antibody, specific to Pentraxin 3/PTX3, Rabbit IgG

Sinónimos

gen relacionado con la pentaxina, rápidamente inducido por IL-1 beta, factor de necrosis tumoral | proteína relacionada con la pentaxina PTX3 | pentraxina 3 | pentraxina 3, larga | pentraxina-3 | gen relacionado con la pentraxina, rápidamente inducido por

Especificaciones y pureza

ExactAb™, validado, sin portador, 1, 0 mg/mL

Especies hospedadoras

Rabbit

Especificidad

Pentraxin 3/PTX3

Inmunógeno

A synthetic peptide derived from human PTX3

Control positivo

WB: Mouse spleen, HEK293, A549, HUVEC, NIH/3T3 and C6 lysates. IHC: Human lung cancer tissue. ICC/IF: HUVEC cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Líquido

Condiciones de almacenamiento de almacenamiento

Conservar a -20°C,Evitar la congelación y descongelación repetidas

Enviado en

Hielera + almohadillas de hielo

Estabilidad y almacenamiento

Conservar a 4°C a corto plazo (1-2 semanas). Conservar a -20°C a largo plazo (12 meses). Tras la recepción, se recomienda realizar alícuotas. Evitar el ciclo de congelación/descongelación.

Imágenes

Western Blot: Pentraxin 3/PTX3 Antibody (Ab121192)

Pentraxin 3/PTX3 Antibody (Ab121192) - Western Blot
All lanes: Pentraxin 3/PTX3 Antibody (Ab121192) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 44 kDa
Exposure time: 12.9 s

IF/ICC: Pentraxin 3/PTX3 Antibody (Ab121192)

Pentraxin 3/PTX3 Antibody (Ab121192) - IF/ICC
Immunofluorescent analysis of PTX3 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Pentraxin 3/PTX3 Antibody (Ab121192) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IHC: Pentraxin 3/PTX3 Antibody (Ab121192)

Pentraxin 3/PTX3 Antibody (Ab121192) - IHC
Immunohistochemical analysis of PTX3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the Pentraxin 3/PTX3 Antibody (Ab121192) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1/50-1/200 ;

• IHC:

  1/50 - 1/100;

• WB:

  1/500-1/1000 ;

Objetivos asociados (humanos)

PTX3 Tbio Pentraxin-related protein PTX3 (0 Activities)

Discover Target: PTX3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)