GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.See COA
Application
Capture Antibody, CLIA, Detection Antibody, ELISA, FIA, FLFIA, GICA, LF
Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Human PLGF Antibody, Monoclonal (5D9-3), is a Capture or Detection antibody could be used for ELISA, CLIA, LF, GICA, FIA. CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE FLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL
Specifications
Product Name
PLGF Mouse mAb - Primary antibody, specific to PGF, Mouse IgG1
25.2 kDa (Light Chain) & 51.1 kDa (Heavy Chain), under reducing conditions; 177.4 kDa, under non-reducing conditions.
Método de purificación
Affinity chromatography
Pares emparejados
[Ab176386] PLGF Mouse mAb as Detection or Capture antibody in ELISA, CLIA, LF, GICA, FIA
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 37°C short term（7 days）Store at 4°C short term（30 days）. Store at -20±5°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176388) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176386) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176388) ·Detection antibody: PLGF Mouse mAb (Ab176386)
Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176386) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176388) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176386) ·Detection antibody: PLGF Mouse mAb (Ab176388)
Binding ELISA - PLGF Mouse mAb (Ab176388) Immobilized Recombinant Human PLGF Protein at 1.0 μg/mL can bind PLGF Mouse mAb (Ab176388) with the EC₅₀ of 38.54 ng/mL.
Aplicación
Aplicación
Dilution info
CLIA
Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
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