RAD17 Antibody - Primary antibody, specific to RAD17, Rabbit IgG

Cat. No.: Ab191358

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human RAD17 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.

Specifications

Product Name

RAD17 Antibody - Primary antibody, specific to RAD17, Rabbit IgG

Sinónimos

CCYC antibody | Cell cycle checkpoint protein antibody | Cell cycle checkpoint protein RAD17 antibody | FLJ41520 antibody | HRAD 17 antibody | hRad17 antibody | R24L antibody | Rad 17 antibody | Rad 24 antibody | RAD1 (S. pombe) homolog antibody | RAD1 ho

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1)

Especies hospedadoras

Rabbit

Especificidad

RAD17

Inmunógeno

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RAD17.

Control positivo

WB: K562, Jurkat and MDA-MB-231 cell lysates. IHC: Human lung cancer tissue. IF/ICC: A549 cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: RAD17 Antibody (Ab191358)

RAD17 Antibody (Ab191358) - Western Blot
All lanes: RAD17 Antibody (Ab191358) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution
Predicted band size: 77 kDa
Observed band size: 79 kDa
Exposure time: 48.5 s

IHC: RAD17 Antibody (Ab191358)

RAD17 Antibody (Ab191358) - IHC
Immunohistochemical analysis of RAD17 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with RAD17 Antibody (Ab191358) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: RAD17 Antibody (Ab191358)

RAD17 Antibody (Ab191358) - IF/ICC
Immunofluorescent analysis of RAD17 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with RAD17 Antibody (Ab191358) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000 ;

• IHC:

  1/100-1/200;

Objetivos asociados (humanos)

RAD17 Tbio Cell cycle checkpoint protein RAD17 (0 Activities)

Discover Target: RAD17

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)