GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.5 mg/mL
ExactAb™, validado, sin portador, 0,5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Human RBCK1 Antibody, Monoclonal (K39S7K), se puede utilizar para WB y así sucesivamente.
Specifications
Product Name
RBCK1 Mouse mAb - Primary antibody, specific to RBCK1, Mouse IgG1
Sinónimos
Chromosome 20 open reading frame 18 antibody | HBV associated factor 4antibody | HBV-associated factor 4 antibody | Heme-oxidized IRP2 ubiquitin ligase 1 antibody | Hepatitis B virus X associated protein 4 antibody | Hepatitis B virus X-associated protein
Especificaciones y pureza
ExactAb™, validado, sin portador, 0,5 mg/mL
Mecanismos bioquímicos y fisiológicos
Actúa como una ubiquitina-proteína ligasa E3, o como parte de un complejo E3, que acepta ubiquitina de enzimas conjugadoras de ubiquitina E2 específicas, como UBE2L3/UBCM4, y luego la transfiere a sustratos. Funciona como E3 ligasa para IREB2 oxidada y ta
Especies hospedadoras
Mouse
Especificidad
RBCK1
Inmunógeno
Recombinant fusion protein of human RBCK1.
Control positivo
WB: Jurkat, HEK293, A375 and A549 cell lysates.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Mouse IgG1
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Líquido
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C,Evitar la congelación y descongelación repetidas
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Conservar a 4°C a corto plazo (1-2 semanas). Conservar a -20°C a largo plazo (12 meses). Tras la recepción, se recomienda realizar alícuotas. Evitar el ciclo de congelación/descongelación.
Imágenes
RBCK1 Mouse mAb (Ab124756) - Western Blot All lanes: RBCK1 Mouse mAb (Ab124756) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 57 kDa Observed band size: 57 kDa The expression molecular weight observed is consistent with what has been described in the literature (PMID: 31209050, PMID: 27006117). The higher MW (57 kDa) band is the ubiquitylated form of RBCK1 (PMID: 31209050). Bands below 57 kDa are cleaved RBCK1 (PMID: 27006117). Exposure time: 65.7 s
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Reseñas
Reseñas de cliente
Preguntas frecuentes
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RBCK1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ25F0622839