RCAS1 Mouse mAb - Primary antibody, specific to RCAS1, Mouse IgG1

Cat. No.: Ab124859

CALIDAD & PUREZA Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

K40051_4H7

Conjugation

Unconjugated

Descripción general

Mouse anti Human RCAS1 Antibody, Monoclonal (K40051_4H7), could be used for WB, IHC and so on.
Application:
WB:1/1000
IHC:1/100
Protein Function:
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

Especificaciones

Product Name

RCAS1 Mouse mAb - Primary antibody, specific to RCAS1, Mouse IgG1

Sinónimos

PDAF antibody | RCAS 1 antibody | RCAS1 antibody | RCAS1_HUMAN antibody | Receptor binding cancer antigen expressed on SiSo cells antibody | Receptor-binding cancer antigen expressed on SiSo cells antibody | EBAG9 | EB9 | PDAF | BAG9 antibody | Cancer ass

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Especies hospedadoras

Mouse

Especificidad

RCAS1

Inmunógeno

Recombinant human EBAG9 (AA 41-210).

Control positivo

WB: SK-OV-3, MCF7, A549, Jurkat, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -80°C,Avoid repeated freezing and thawing

Enviado en

Dry ice packs + Cold packs

Estabilidad y almacenamiento

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: RCAS1 Mouse mAb (Ab124859)

RCAS1 Mouse mAb (Ab124859) - Western Blot
All lanes: RCAS1 Mouse mAb (Ab124859) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 31 kDa
Exposure time: 36.5 s

IHC: RCAS1 Mouse mAb (Ab124859)

RCAS1 Mouse mAb (Ab124859) - IHC
IHC analysis of human brain tissue by RCAS1 Mouse mAb (Ab124859). IHC was performed using sections of the formalin-fixed paraffin-embedded human brain tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RCAS1 Mouse mAb (Ab124859) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glial cells and neuronal cells are positively stained at the cytoplasm.

Aplicación

Aplicación

Dilution info

WB

1/1000

IHC

1/100

Objetivos asociados (humanos)

EBAG9 Tbio Receptor-binding cancer antigen expressed on SiSo cells (0 Activities)

Discover Target: EBAG9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RCAS1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.