GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human ATG3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function： E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Specifications
Product Name
Recombinant ATG3 Antibody - Primary antibody, specific to ATG3, Rabbit IgG
Sinónimos
APG3-like | Autophagy-related protein 3 | EC 2.3.2.- | hApg3 | Protein PC3-96 | 2610016C12Rik antibody | Apg 3 antibody | APG3 antibody | APG3 autophagy 3 like antibody | APG3 like antibody | APG3, S. cerevisiae, homolog of antibody | APG3-like antibody |
Especificaciones y pureza
Recombinant, ExactAb™, Validated, See COA
Especies hospedadoras
Rabbit
Especificidad
ATG3
Inmunógeno
A synthetic peptide derived from human ATG3 (AA 2-20)
Control positivo
WB: K562, HeLa, THP-1, Jurkat, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
Tipo de cadena ligera
kappa
SDS-PAGE
150kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant ATG3 Antibody (Ab089994) - Western Blot All lanes: Recombinant ATG3 Antibody (Ab089994) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 49.5 s
Recombinant ATG3 Antibody (Ab089994) - IF/ICC Immunocytochemistry analysis of ATG3 (green) in HeLa using Recombinant ATG3 Antibody (Ab089994) and DAPI (blue).
Recombinant ATG3 Antibody (Ab089994) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant ATG3 Antibody (Ab089994). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F0809165
