GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.7 mg/mL
ExactAb™, Validated, Recombinant, 0.7 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human Bmi1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Specifications
Product Name
Recombinant Bmi1 Antibody - Primary antibody, specific to Bmi1, Rabbit IgG
Sinónimos
Polycomb group RING finger protein 4 | RING finger protein 51 | B lymphoma Mo MLV insertion region (mouse) antibody | B lymphoma Mo MLV insertion region 1 homolog antibody | Bmi 1 antibody | BMI1 antibody | BMI1 polycomb ring finger oncogene antibody | BM
Especificaciones y pureza
ExactAb™, Validated, Recombinant, 0.7 mg/mL
Especies hospedadoras
Rabbit
Especificidad
Bmi1
Inmunógeno
Recombinant protein of human Bmi1
Control positivo
WB: K562, HeLa, U-2 OS, MCF7, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified
Concentración
0.7 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant Bmi1 Antibody (Ab091398) - Western Blot All lanes: Recombinant Bmi1 Antibody (Ab091398) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 40 kDa Exposure time: 35.5 s
Recombinant Bmi1 Antibody (Ab091398) - IF/ICC Immunocytochemistry analysis of BMI1 (green) in HeLa using Recombinant Bmi1 Antibody (Ab091398) and DAPI (blue).
Aplicación
Aplicación
Dilution info
IHC
1/50 - 1/100
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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