Anticuerpo recombinante CDKN2A/p14ARF - Primary antibody, specific to CDKN2A, Rabbit IgG

Cat. No.: Ab095833

GRADE & PURITY Recombinant, ExactAb™, Validated, See COA

Application

IF/ICC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Anticuerpo de conejo anti CDKN2A/p14ARF humano, recombinante, puede usarse para WB, ICC, IF, etc.
Aplicación:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Relevancia
El gen para CDK2NA genera varios transcritos/proteínas que difieren entre sí en sus primeros exones. Tres de estos transcritos se generan por splicing alternativo (isoforma 1 a.k.a p16INK4A, isoforma 2 e isoforma 3 a.k.a p12), dos de las cuales se sabe que funcionan como inhibidores de la quinasa CDK4. Otro transcrito que se genera a partir de este gen contiene un marco de lectura alternativo (ARF), con el primer exón situado 20kb aguas arriba del resto del gen (isoforma 4 también conocida como p14ARF, p19ARF, ARF). A pesar de las diferencias estructurales y algunas funcionales, todas las proteínas codificadas por el gen CDKN2A están implicadas en el control del ciclo celular G1.

Specifications

Product Name

Anticuerpo recombinante CDKN2A/p14ARF - Primary antibody, specific to CDKN2A, Rabbit IgG

Sinónimos

Anticuerpo ARF | Anticuerpo CDK4 inhibidor p16 INK4 | Anticuerpo CDK4I | Anticuerpo CDKN2 | Anticuerpo CDKN2A | Anticuerpo regulador negativo del ciclo celular beta | Anticuerpo CMM2 | Anticuerpo inhibidor A de la quinasa 4 dependiente de ciclina | Anticu

Especificaciones y pureza

Recombinante, ExactAb™, Validado, Ver COA

Especies hospedadoras

Rabbit

Especificidad

CDKN2A

Inmunógeno

A synthetic peptide derived from human CDKN2A/p14ARF (AA 75-132).

Control positivo

WB: HEK293 and LNCaP cell lysates. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Almacenamiento y envío

Forma

Líquido

Condiciones de almacenamiento de almacenamiento

Conservar a -20°C,Evitar la congelación y descongelación repetidas

Enviado en

Hielera + almohadillas de hielo

Estabilidad y almacenamiento

Conservar a 4°C a corto plazo (1-2 semanas). Conservar a -20°C a largo plazo (24 meses). Tras la recepción, se recomienda realizar alícuotas. Evitar el ciclo de congelación/descongelación.

Imágenes

Western Blot: Recombinant CDKN2A/p14ARF Antibody (Ab095833)

Recombinant CDKN2A/p14ARF Antibody (Ab095833) - Western Blot
All lanes: Recombinant CDKN2A/p14ARF Antibody (Ab095833) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa
Exposure time: 100.0 s

IF/ICC: Recombinant CDKN2A/p14ARF Antibody (Ab095833)

Recombinant CDKN2A/p14ARF Antibody (Ab095833) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant CDKN2A/p14ARF Antibody (Ab095833) at 1/50 dilution.

Aplicación

Aplicación

Dilution info

IF/ICC

1/50-1/200

WB

1/500-1/1000

Objetivos asociados (humanos)

CDKN2A Tbio Tumor suppressor ARF (0 Activities)

Discover Target: CDKN2A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CDKN2A/p14ARF Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.