GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Recombinante, ExactAb™, Validado, 0,5 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Anticuerpo de conejo anti ENPP5 humano, recombinante, puede usarse para WB, ICC, IF, etc. Aplicación: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Función de la Proteína： Puede desempeñar un papel en la comunicación celular neuronal. Carece de actividad nucleótido pirofosfatasa y lisofolipasa D.
Specifications
Product Name
Anticuerpo recombinante ENPP5 - Primary antibody, specific to ENPP5, Rabbit IgG
KLH-conjugated synthetic peptide encompassing a sequence within human ENPP5.
Control positivo
WB: HEK293, MCF7, HeLa, Hep G2, NIH/3T3 and C6 cell lysates. ICC/IF: COS7 cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Immunogen affinity purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Líquido
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C,Evitar la congelación y descongelación repetidas
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Almacenar a 4℃ a corto plazo (1-2 semanas). Almacene en -20℃ a largo plazo (12 meses). Tras la recepción, se recomienda alícuota. Evite el ciclo de congelación/descongelación.
Imágenes
Recombinant ENPP5 Antibody (Ab101745) - Western Blot All lanes: Recombinant ENPP5 Antibody (Ab101745) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 60.0 s
Recombinant ENPP5 Antibody (Ab101745) - Western Blot All lanes: Recombinant ENPP5 Antibody (Ab101745) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa Observed band size: 51 kDa Exposure time: 100.0 s
Recombinant ENPP5 Antibody (Ab101745) - IF/ICC Immunofluorescent analysis of ENPP5 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 mintes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Recombinant ENPP5 Antibody (Ab101745) in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Aplicación
Aplicación
Dilution info
IF/ICC
1/50-1/200
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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