Recombinant MSH2 Antibody - Primary antibody, specific to MSH2, Rabbit IgG

Cat. No.: Ab116092

GRADE & PURITY Recombinant, Validated, ExactAb™, 1.2 mg/mL

Application

IHC

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Clone Number

MyR1-MSH2

Conjugation

Unconjugated

Descripción general

Rabbit anti Human MSH2 Antibody, Recombinant (MyR1-MSH2), could be used for IHC and so on.

Application

IHC: 1/100-1/200

Protein Function

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Specifications

Product Name

Recombinant MSH2 Antibody - Primary antibody, specific to MSH2, Rabbit IgG

Sinónimos

MutS protein homolog 2 | mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) | MSH2 | LCFS2 | COCA 1 antibody | BAT26 antibody | COCA1 antibody | DNA mismatch repair protein Msh2 antibody | FCC 1 antibody | HNPCC1mutS (E. coli) homolog 2 (colon ca

Especificaciones y pureza

ExactAb™, Validated, Recombinant, 1.2 mg/mL

Especies hospedadoras

Rabbit

Especificidad

MSH2

Inmunógeno

Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).

Control positivo

IHC: Human colorectal cancer tissue.

Conjugación

Unconjugated

Grado

ExactAb™, Recombinant, Validated

Isotipo

Rabbit IgG

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

1.2 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Avoid repeated freezing and thawing

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 2-8°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

IHC: Recombinant MSH2 Antibody (Ab116092)

Recombinant MSH2 Antibody (Ab116092) - IHC
Immunohistochemistry analysis of paraffin-embedded human intestinal cancer tissue labelling MSH2 with Recombinant MSH2 Antibody (Ab116092) at 1/100 dilution. The section was incubated with Recombinant MSH2 Antibody (Ab116092) for 30 mins at room temperature. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was used for antigen retrieval.​

Aplicación

• Aplicación: Dilution info
• IHC:

  1/100 - 1/200;

Objetivos asociados (humanos)

MSH2 Tbio DNA mismatch repair protein Msh2 (0 Activities)

Discover Target: MSH2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción