GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human Myosin light chain kinase/MLCK Antibody, Recombinant, could be used for WB, IHC and so on.
Specifications
Product Name
Recombinant Myosin light chain kinase/MLCK Antibody - Primary antibody, specific to MYLK, Rabbit IgG
Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial
Especies hospedadoras
Rabbit
Especificidad
MYLK
Inmunógeno
A synthetic peptide derived from human MLCK (AA 1850-1914)
Control positivo
WB: HUVEC, A431 and HEK293 cell lysates. IHC: Human colon tissue.IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) - Western Blot All lanes: Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 211 kDa Observed band size: 120 kDa Exposure time: 15.8 s
Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) - IHC Immunohistochemical analysis of paraffin-embedded human colon, using Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903).
Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) - IF/ICC IF analysis of Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Myosin light chain kinase/MLCK Antibody (Ab116903) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
IHC
1/100 - 1/200
WB
1/1000 - 1/2000
IF/ICC
1:500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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