GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.2 mg/mL
ExactAb™, Validated, Recombinant, 0.2 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human pan Cadherin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500 - 1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Relevance
Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.
Specifications
Product Name
Recombinant pan Cadherin Antibody - Primary antibody, specific to CDH1; CDH3; CDH2, Rabbit IgG
A synthetic peptide derived from human pan Cadherin (AA 867-882)
Control positivo
WB: Mouse heart, Mouse brain, HeLa, A431, MCF7, A549 and C6 lysates. IHC: Human cholangiocarcinoma tissue. ICC/IF: MCF7 cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Antigen affinity purified
Concentración
0.2 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant pan Cadherin Antibody (Ab120139) - Western Blot All lanes: Recombinant pan Cadherin Antibody (Ab120139) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 100-140 kDa Exposure time: 4.1 s
Recombinant pan Cadherin Antibody (Ab120139) - Western Blot All lanes: Recombinant pan Cadherin Antibody (Ab120139) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 97 kDa Observed band size: 124 kDa Exposure time: 12.2 s
Recombinant pan Cadherin Antibody (Ab120139) - IF/ICC Immunocytochemistry analysis of pan Cadherin (green) in MCF7 using Recombinant pan Cadherin Antibody (Ab120139), and cells were counterstained with DAPI (blue).
Recombinant pan Cadherin Antibody (Ab120139) - IHC Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant pan Cadherin Antibody (Ab120139). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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