GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human SDHB Antibody, Recombinant, could be used for WB, IP and so on.
Specifications
Product Name
Recombinant SDHB Antibody - Primary antibody, specific to SDHB, Rabbit IgG
Sinónimos
CWS2 antibody | DHSB_HUMAN antibody | FLJ92337 antibody | Ip antibody | Iron sulfur subunit antibody | Iron sulfur subunit of complex II antibody | Iron-sulfur subunit of complex II antibody | mitochondrial antibody | PGL 4 antibody | PGL4 antibody | SDH
Especificaciones y pureza
Recombinant, ExactAb™, Validated, See COA
Mecanismos bioquímicos y fisiológicos
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Especies hospedadoras
Rabbit
Especificidad
SDHB
Inmunógeno
A synthetic peptide derived from human SDHB (AA 1-280).
Control positivo
WB: Mouse slpeen, Rat spleen, HeLa, Jurkat and C6 lysates. IP: HeLa lysates. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant SDHB Antibody (Ab126854) - Western Blot All lanes: Recombinant SDHB Antibody (Ab126854) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 32 kDa Observed band size: 28, 24 kDa The band observed at 24 kDa could potentially be a cleaved form of SDHB due to the presence of a 28 amino acid transit peptide. Exposure time: 12.4 s
Recombinant SDHB Antibody (Ab126854) - IP Immunoprecipitate (IP) analysis using Recombinant SDHB Antibody (Ab126854) at 1/50 dilution. (WB with Recombinant SDHB Antibody (Ab126854) at 1/1000 dilution).
Recombinant SDHB Antibody (Ab126854) - IF/ICC IF analysis of Recombinant SDHB Antibody (Ab126854) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SDHB Antibody (Ab126854) at 1/300 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
WB
1/1000 - 1/2000
IP
1/20-1/50
IF/ICC
1/300
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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