GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human STAT4 Antibody, Recombinant, could be used for WB and so on.
Specifications
Product Name
Recombinant STAT4 Antibody - Primary antibody, specific to STAT4, Rabbit IgG
Sinónimos
DPMC | SLEB11 | signal transducer and activator of transcription 4 | STAT4 | Signal transducer and activator of transcription 4 antibody | SLEB11 antibody | STAT4 antibody | STAT4_HUMAN antibody
Especificaciones y pureza
Recombinant, ExactAb™, Validated, See COA
Mecanismos bioquímicos y fisiológicos
Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
Especies hospedadoras
Rabbit
Especificidad
STAT4
Inmunógeno
A synthetic peptide derived from human STAT4 (AA 700-748)
Control positivo
WB: HeLa, Jurkat, Hep G2, MCF7 and MOLT-4 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
See COA
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant STAT4 Antibody (Ab129436) - Western Blot All lanes: Recombinant STAT4 Antibody (Ab129436) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 86 kDa Observed band size: 48, 86 kDa STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors (PMID:22383755). The DNA-binding domain can bind to DNA which affects its migration in Western blot experiments. Exposure time: 86.5 s
Recombinant STAT4 Antibody (Ab129436) - IF/ICC IF analysis of Recombinant STAT4 Antibody (Ab129436) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant STAT4 Antibody (Ab129436) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
WB
1/1000 - 1/2000
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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