GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.2 mg/mL
ExactAb™, Validated, Recombinant, 0.2 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Rabbit anti Human Wnt5a Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
Specifications
Product Name
Recombinant Wnt5a Antibody - Primary antibody, specific to WNT5A, Rabbit IgG
Sinónimos
WNT5A | Protein Wnt-5a | hWNT 5A antibody | hWNT5A antibody | Protein Wnt 5a antibody | Protein Wnt-5a antibody | Protein Wnt5a antibody | Wingless type MMTV integration site family member 5A antibody | Wnt 5a antibody | WNT 5A protein antibody | WNT 5A p
Especificaciones y pureza
ExactAb™, Validated, Recombinant, 0.2 mg/mL
Especies hospedadoras
Rabbit
Especificidad
WNT5A
Inmunógeno
A synthetic peptide derived from human Wnt5a (AA 340-354).
Control positivo
WB: A431 and HeLa cell lysates. ICC/IF: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Recombinant, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Immunogen affinity purified
Concentración
0.2 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Recombinant Wnt5a Antibody (Ab134765) - Western Blot All lanes: Recombinant Wnt5a Antibody (Ab134765) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 6.3 s
Recombinant Wnt5a Antibody (Ab134765) - Western Blot All lanes: Recombinant Wnt5a Antibody (Ab134765) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 42 kDa Observed band size: 45 kDa Exposure time: 1.0 s
Recombinant Wnt5a Antibody (Ab134765) - IF/ICC Immunocytochemistry analysis of Wnt5a (green) in HeLa using Recombinant Wnt5a Antibody (Ab134765), and cells were counterstained with DAPI (blue).
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ23F1001324
