CALIDAD & PUREZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Carrier Free, ExactAb™, Validated, 1.0 mg/mL Carrier Free,ExactAb™,Validated para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.
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Almacenamiento y envío
Store at -80°C,Avoid repeated freezing and thawing Envío Dry ice packs + Cold packs. Consulte el COA específico del lote para conocer las especificaciones exactas.
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Documentos de calidad
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Prueba documental
Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.
Descripción general
Mouse anti Human SCAMP5 Antibody, Monoclonal (K06322_12G11), could be used for WB, IHC, IP and so on. Application: WB: 1/1000 IHC: 1/200 IP: 1/100 Protein Function Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.
Especificaciones
Product Name
SCAMP5 Mouse mAb - Primary antibody, specific to SCAMP5, Mouse IgG1
Store at -80°C,Avoid repeated freezing and thawing
Enviado en
Dry ice packs + Cold packs
Estabilidad y almacenamiento
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
SCAMP5 Mouse mAb (Ab126689) - Western Blot All lanes: SCAMP5 Mouse mAb (Ab126689) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 26 kDa Observed band size: 24 kDa Exposure time: 100.0 s
SCAMP5 Mouse mAb (Ab126689) - IHC IHC analysis of cerebellum tissue by SCAMP5 Mouse mAb (Ab126689). IHC was performed using sections of the formalin-fixed paraffin-embedded cerebellum tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with SCAMP5 Mouse mAb (Ab126689) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Cytoplasm of cells in granular layer and in molecular layer are positively stained.
SCAMP5 Mouse mAb (Ab126689) - IP Immunoprecipitation was performed by incubation of 2.5 µg of SCAMP5 Mouse mAb (Ab126689) with mouse brain lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. SCAMP5 Mouse mAb (Ab126689) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: Mouse brain lysate Lane 2: SCAMP5 immunoprecipitated from mouse brain lysate by SCAMP5 Mouse mAb (Ab126689) Lane 3: The same as Lane 2 but Mouse IgG was used as IgG isotype control antibody Result: SCAMP5 Mouse mAb (Ab126689) can immunoprecipitate SCAMP5.
Aplicación
Aplicación
Dilution info
WB
1/1000
IP
1/100
IHC
1/200
Documentación
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