SIRT5 Mouse mAb - Primary antibody, specific to SIRT5, Rabbit IgG

Cat. No.: Ab127693

GRADE & PURITY See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

OTI2F10

Conjugation

Unconjugated

Descripción general

Mouse anti Human SIRT5 Antibody, Monoclonal (OTI2F1), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

SIRT5 Mouse mAb - Primary antibody, specific to SIRT5, Rabbit IgG

Sinónimos

EC 2.3.1.- | Regulatory protein SIR2 homolog 5 | SIR2-like protein 5 | NAD dependent deacetylase sirtuin 5antibody | NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial antibody | NAD dependent protein deacylase sirtuin 5 mitochond

Especificaciones y pureza

See COA

Mecanismos bioquímicos y fisiológicos

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of

Especies hospedadoras

Mouse

Especificidad

SIRT5

Inmunógeno

Recombinant full length protein of human SIRT5 produced in HEK293.

Control positivo

WB: HEK293T transfected with pCMV6-ENTRY SIRT5 cDNA for 48 hrs, HepG2, HeLa, A549, COS7, PC12, MCF7 lysates. IHC: Human Kidney tissue. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY SIRT5. Flow Cytometry: HEK293T cells transfected with pCMV6-

Conjugación

Unconjugated

Isotipo

Rabbit IgG

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/100;

• IHC:

  1/150

• IF/ICC:

  1:100 ;

Objetivos asociados (humanos)

SIRT5 Tchem NAD-dependent protein deacylase sirtuin-5, mitochondrial (0 Activities)

Discover Target: SIRT5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

SIRT5 Tchem NAD-dependent protein deacylase sirtuin-5, mitochondrial (1056 Activities)

Discover Target: SIRT5

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción