SMAD5 Mouse mAb - Primary antibody, specific to SMAD5, Mouse IgG1

Cat. No.: Ab128237
Disponible para pedir
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 1.6 mg/mL
Application
Flow Cytometry, IF/ICC, WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG1
Clone Number
4B10-B10-B6
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μl
Ab128237-10μl
2
99,90US$
50μl
Ab128237-50μl
1
259,90US$
100μl
Ab128237-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
419,90US$
1ml
Ab128237-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
3.399,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 1.6 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Mouse anti Human SMAD5 Antibody, Monoclonal (4B10-B10-B6), could be used for WB, IF/ICC, Flow and so on.
Application:
WB: 1/500-1/1000
IF/ICC: 1/50-1/200    
Flow: 1/50-1/100
Protein Function:
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Specifications

Product Name
SMAD5 Mouse mAb - Primary antibody, specific to SMAD5, Mouse IgG1
Sinónimos
Dwfc | hSmad5 | JV5-1 | JV5-1DKFZp781O1323 | MAD homolog 5 | MAD, mothers against decapentaplegic homolog 5 (Drosophila) | MADH5 | MADH5mothers against decapentaplegic homolog 5 | mothers against decapentaplegic homolog 5 | mothers against decapentaplegic
Especificaciones y pureza
ExactAb™, Validated, 1.6 mg/mL
Especies hospedadoras
Mouse
Especificidad
SMAD5
Inmunógeno
Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli. (AA 1-465).
Control positivo
WB: HeLa, SK-N-SH, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells. Flow: Jurkat cells.
Conjugación
Unconjugated
Grado
ExactAb™, Validated
Isotipo
Mouse IgG1
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
1.6 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes

Recombinant SMAD5 Antibody (Ab128237) - Western Blot
All lanes: Recombinant SMAD5 Antibody (Ab128237) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 1.0 s

Recombinant SMAD5 Antibody (Ab128237) - IF/ICC
Immunocytochemistry analysis of Smad5 in HeLa cells using Recombinant SMAD5 Antibody (Ab128237).

Recombinant SMAD5 Antibody (Ab128237) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells stained with Recombinant SMAD5 Antibody (Ab128237) (red). Black line histogram represents the isotype control, normal mouse IgG.

Aplicación
AplicaciónDilution info
WB

1/500-1/1000

IF/ICC

1/50 - 1/200

Flow Cytometry

1/50-1/100

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
SMAD5 Tbio Mothers against decapentaplegic homolog 5 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeFechaArticulo
ZJ24F0404707Certificate of AnalysisFeb 10, 2026 Ab128237
ZJ24F0404708Certificate of AnalysisFeb 10, 2026 Ab128237
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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