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TNF alpha Mouse mAb - Primary antibody, specific to Human TNF alpha

Cat. No.: Ab215301
COA
TNF alpha Mouse mAb - by Aladdin Scientific
Disponible para pedir
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Detection Antibody, ELISA
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
C08/3E2
Conjugation
Unconjugated
★
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μg
Ab215301-10μg
4
75,90US$
50μg
Ab215301-50μg
3
223,90US$
100μg
Ab215301-100μg
2
359,90US$
1mg
Ab215301-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
1.023,90US$
Enter a quantity for the sizes you want to add.
Bulk / Custom
♡ Lista de deseos ⇅ Añadir para comparar ⎙ Print page
SDS
Safety data
COA
Certificate
DS
Datasheet
SP
Spec sheet
Descripción general Specifications Almacenamiento y envío Imágenes Aplicación Documentation Advanced Data Objetivos asociados (humanos) Certificados (CoA, COO, BSE/TSE y tabla de análisis) Preguntas frecuentes y artículos Calculadoras de soluciones Reseñas
🧪

Why this grade

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Animal Free,Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Mouse anti Human TNF alpha Antibody, clone C08/3E2, could be used for a Detection Antibody in sandwich ELISAs

Specifications

Product Name
TNF alpha Mouse mAb - Primary antibody, specific to Human TNF alpha
Sinónimos
APC1 antibody | APC1 protein antibody | Cachectin antibody | DIF antibody | Differentiation inducing factor antibody | Macrophage cytotoxic factor antibody | Tnf antibody | TNF superfamily member 2 antibody | TNF superfamily, member 2 antibody | TNF, macr
Especificaciones y pureza
Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL
Mecanismos bioquímicos y fisiológicos
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is i
Especies hospedadoras
Mouse
Especificidad
Human TNF alpha
Inmunógeno
Recombinant protein corresponding to Human TNF alpha
Conjugación
Unconjugated
Grado
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Pares emparejados
[Ab156547] TNF alpha Mouse mAb, Capture Antibody
Concentración
1.0 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Imágenes

Sandwich ELISA - TNF alpha standard curve
Sandwich ELISA detection of Recombinant Human TNF-α Protein (rp156007) using TNF alpha Mouse mAb (Ab156547) as capture antibody at concentration of 1.0 µg/mL. Biotinylated TNF alpha Mouse mAb (Ab215301) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.97 ng/mL - 1000.0 ng/mL

Indirect ELISA - TNF alpha standard curve
Immobilized Recombinant Human TNF-α Protein (rp156007) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with TNF alpha Mouse mAb (Ab156547). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 59.41 ng/mL

Aplicación
AplicaciónDilution info
ELISA

1.0 µg/mL as a Detection antibody in ELISA

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
TNF Tclin Tumor necrosis factor (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Preguntas frecuentes y artículos
TNF Signaling Pathway: Receptor Layering Structure, Complex Conversion Mechanisms, and the Inflammation–Death Effector Network
Categories: Technical articles
Tags:
TNF Apoptosis inflammatory signaling MAPK TNFR1 TNFR2 RIPK1 necroptosis
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Animal Free grade guide → View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →
Bulk & Custom Packaging Need larger quantities or custom specifications? Contact our team for a tailored quote.

View related series

Click to view target information: TNF
 Accession#:P01375 (26) Gene ID:7124 (26) Tnf (32) TNF alpha (11)

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