Anticuerpo TXNDC4 - Primary antibody, specific to TXNDC4, Rabbit IgG

Cat. No.: Ab133085

CALIDAD & PUREZA Carrier Free, ExactAb™, Validated, 0.2 mg/mL

Application

IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Anticuerpo de conejo anti TXNDC4 humano, policlonal, se puede utilizar para WB, IHC y así sucesivamente.
Aplicación:
WB: 1/500-1/1000
IHC: 1/50-1/100
Función de la proteína
Media la retención dependiente de tiol en la vía secretora temprana, formando disulfuros mixtos con proteínas sustrato a través de su motivo conservado CRFS. Inhibe la actividad de canal de calcio de ITPR1. Puede desempeñar un papel en el control del plegamiento oxidativo de proteínas en el retículo endoplásmico. Necesaria para retener ERO1L y ERO1LB en el retículo endoplásmico.

Especificaciones

Product Name

Anticuerpo TXNDC4 - Primary antibody, specific to TXNDC4, Rabbit IgG

Sinónimos

TXNDC4 | Anticuerpo contra la proteína 44 kDa residente en el retículo endoplásmico | Anticuerpo contra la proteína 44 kDa residente en el retículo endoplásmico | Anticuerpo contra la proteína ERp44 residente en el retículo endoplásmico | Anticuerpo contr

Especificaciones y pureza

ExactAb™, validado, sin portador, 0,2 mg/mL

Especies hospedadoras

Rabbit

Especificidad

TXNDC4

Inmunógeno

A synthetic peptide derived from human TXNDC4 (AA 392-406)

Control positivo

WB: Raji, A549, HeLa, Hep G2, MDA-MB-231, K562, A431, HEK293, THP-1, NIH/3T3 and C6 cell lysates. IHC: Human liver cancer tissue.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

0.2 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Líquido

Condiciones de almacenamiento de almacenamiento

Conservar a -20°C,Evitar la congelación y descongelación repetidas

Enviado en

Hielera + almohadillas de hielo

Estabilidad y almacenamiento

Conservar a 4°C a corto plazo (1-2 semanas). Conservar a -20°C a largo plazo (24 meses). Tras la recepción, se recomienda realizar alícuotas. Evitar el ciclo de congelación/descongelación.

Imágenes

Western Blot: TXNDC4 Antibody (Ab133085)

TXNDC4 Antibody (Ab133085) - Western Blot
All lanes: TXNDC4 Antibody (Ab133085) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 47 kDa
Observed band size: 42 kDa
Exposure time: 200.0 s

IHC: TXNDC4 Antibody (Ab133085)

TXNDC4 Antibody (Ab133085) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using TXNDC4 Antibody (Ab133085). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Aplicación

Aplicación

Dilution info

IHC

1/50 - 1/100

WB

1/500-1/1000

Objetivos asociados (humanos)

ERP44 Tbio Endoplasmic reticulum resident protein 44 (0 Activities)

Discover Target: ERP44

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TXNDC4 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.