Acetate Kinase, from Escherichia coli, CAS No.9027-42-3

CAS: 9027-42-3 Cat. No.: A293614 EC Number: 232-818-0

GRADE & PURITY EnzymoPure™, lyophilized powder, ≥150 units/mg protein (biuret)

Accession #

P0A6A3

Overview

Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily.It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain.
Application
Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies.
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation.
Physical form
Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol

Specifications

Product Name

Acetate Kinase, from Escherichia coli, CAS No.9027-42-3

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, lyophilized powder, ≥150 units/mg protein (biuret)

Biochemical and Physiological Mechanisms

Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propan

Accession #

P0A6A3

CAS

9027-42-3

Enzyme Commission Number

2.7.2.1 (BRENDA, IUBMB)

Molecule Type

Enzyme

Storage and Shipping

Concentration

lyophilized powder, ≥150 units/mg protein (biuret)

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Unit definition

Under pH 7.6 and 25 ℃ conditions, one unit can phosphorylate 1.0 μ mol of acetic acid into acetyl phosphate per minute.