GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation.
Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzhei
Host species
Mouse
Specificity
Human Cathepsin D
Immunogen
Recombinant protein corresponding human Cathepsin D
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Light Chain Type
kappa
SDS-PAGE
150.0 KDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Cathepsin D Mouse mAb (Ab215111) - Western Blot All lanes: Cathepsin D Mouse Antibody (Ab215111) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 45 kDa Observed band size: 29 kDa Exposure time: 60.0 s
Cathepsin D Mouse mAb (Ab215111) - Flow Cytometry Overlay histogram showing MCF-7 cells stained with Cathepsin D Mouse mAb (Ab215111) (Red) at 1.25 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557) was used as an isotype control (Blue).
Cathepsin D Mouse mAb (Ab215111) - IF IF analysis of Cathepsin D Mouse mAb (Green) in MCF-7 cells. The cells were fixed with 4% PFA for 15 minutes and permeabilized with 0.3% Triton X-100 for 15 minutes at RT. Then the cells were stained with Cathepsin D Mouse mAb (Ab215111) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ab181952) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Application
Application
Dilution info
IF/ICC
1:1000
WB
1/500-1/5000
Flow Cytometry
1/200–1/5000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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