Creatinine deiminase (CNI), CAS No.37289-15-9

CAS: 37289-15-9 Cat. No.: C774080 Molecular Weight: ~260 kDa EC Number: 253-449-1

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥20 U/mg enzyme powder; ≥100 U/mg protein

Accession #

O32386

Expression system

E. coli

Bioactivity

≥20 U/mg enzyme powder; ≥100 U/mg protein

Overview

Creatinine Deiminase (CNI, EC 3.5.4.21) is a hydrolase that specifically catalyzes the ring-opening hydrolysis of creatinine, producing N-methylhydantoin and ammonia (NH₃). This enzyme is commonly found in microorganisms and is widely used in clinical diagnostics for the detection of creatinine levels. It serves as a key enzymatic component in vitro diagnostic reagents for renal function assessment. 

Reaction

Properties

Source: Microorganism 
Isoelectric point: 5.8 
Michaelis constant: 6.6×10^-5 M (Creatinine) 
Optimum pH: 8.0                     Fig. 1 
Optimum temperature: 40~55℃                     Fig. 3 
pH Stability: 7.5~9.5 (30℃, 20hr)                     Fig. 2 
Thermal stability: <60℃ (pH 7.5, 60min)                     Fig. 4 
Inhibitors: Co2+, Cu2+, Fe3+, Ni2+, Zn2+, SDS 
Effect of various chemicals:                     Table 1 

Specifications

Product Name

Creatinine deiminase (CNI), CAS No.37289-15-9

Synonyms

Creatinine deaminase | Creatinine iminohydrolase | Creatinine hydrolase

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥20 U/mg enzyme powder; ≥100 U/mg protein

Bioactivity

≥20 U/mg enzyme powder; ≥100 U/mg protein

Accession #

O32386

SDS-PAGE

46 kDa (SDS-PAGE)

CAS

37289-15-9

Enzyme Commission Number

EC 3.5.4.21

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥20 U/mg enzyme powder; ≥100 U/mg protein

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (15 days). Store at -20℃ long term (2 years). Upon delivery aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

One unit will cause the formation of one micromole of ammonia per min at pH 7.5 at 37℃.