Cell micrometry experiments
Cell micrometry experiments
Cell micrometry experiments are experiments in which the size of cells is measured, and the method of this experiment is mainly to measure the size of cells with a microscopic micrometer. Currently, the main method of measurement is microscopic micrometer to measure the cell microscopic size.
Principle
The basic principle of cell micrometry experiments is carried out with the aid of a microscopic micrometer. The eyepiece micrometer is a circular slide placed in the eyepiece with a 5 mm or 10 mm long scale in the center, which can be divided into 50 or 100 frames. The actual length of each frame varies with the magnification of the objective lens. The objective micrometer is a slide with a circular 1 mm or 2 mm micrometer in the center that is divided into 100 or 200 compartments, each of which has an actual length of 0.01 mm. When measuring cell size, the actual length of each compartment of the eyepiece micrometer is first verified with the objective micrometer, and then the eyepiece micrometer is used to measure the specimen. Conversion formula: eyepiece per cell length (μm) = number of cells of the table scale / number of cells of the eyepiece x 10 μm
Operation method
Microscopic micrometer measuring cell microsize experiment
Principle
The basic principle of the microscope micrometer experiment for measuring cell microscopic size is to use an eyepiece micrometer in conjunction with an objective micrometer: the eyepiece micrometer is a circular slide placed in the eyepiece, with a 5 mm or 10 mm scale in the center, which can be divided into 50 or 100 compartments. The actual length of each frame changes depending on the magnification of the objective lens. The objective micrometer is a slide with a circular 1 mm or 2 mm micrometer in the center that is divided into 100 or 200 compartments, each of which has an actual length of 0.01 mm. When measuring cell size, the actual length of each compartment of the eyepiece micrometer is first verified with the objective micrometer, and then the eyepiece micrometer is used to measure the specimen. Conversion formula: eyepiece per cell length (μm) = number of cells of the table scale / number of cells of the eyepiece x 10 μm
Materials and Instruments
Equipment: Move The basic process of microscopic micrometer measurement of cell microscopic size can be divided into the following steps: Caveat 1. Cells in single-cell suspensions should be dispersed as much as possible; cell length measurements will be affected if cells overlap each other. 2. Before aligning the zero line on the left side of the stage and eyepiece, the microscope focus should be adjusted as much as possible to minimize the error. 3. The actual length of each cell of the eyepiece should be recalculated when the measurement is made with an objective lens of a different magnification. 4. Not less than 10 cells should be measured and the average value should be taken at the end. For more product details, please visit Aladdin Scientific website.
① Cultured single cell suspension (104 cells/ml)
② Pipette
Straws ③ Gel caps
④ Centrifuge tube
⑤ Alcohol cotton balls
⑥ Alcohol lamp
⑦ Absorbent paper
⑧ Slide
⑨ Coverslip
⑩ Objective microscope
⑪ Eyepiece microscope
⑫ General optical microscope
Reagents: ① Iodine solution
① Iodine solution
A. Remove the upper lens of the microscope eyepiece, mount the eyepiece scale downward on the focal plane of the eyepiece, screw on the upper lens, and insert the eyepiece into the lens barrel.
B. Place the table ruler scale upward on the carrier stage, and adjust the focus at low magnification to make the object ruler scale clear.
C. Rotate the eyepiece and move the object ruler to make the two rulers parallel and the zero line on the left is aligned. Find the other alignment line of the two rulers from left to right.
D. Record the number of squares of the eyepiece and the table ruler between the two overlapping lines, and convert the actual length of each square of the eyepiece according to the formula.
E. Add a drop of suspension of cultured cells in the center of the slide, cover the coverslip, and add iodine solution from one side of the slide, and suck out part of the liquid on the other side of the slides with blotting paper to make a slide of the cultured cells.
F. Remove the table ruler, and place the cultured cells on the carrier table. slide on the carrier table.
G. Move the cell slide and rotate the eyepiece so that the eyepiece overlaps the diameter of the cell to be measured, and record the number of frames of the eyepiece.