Modeling experiments in an animal model of membranous labyrinthine effusion
Modeling experiments in an animal model of membranous labyrinthine effusion
Meniere disease (MED) is a common clinical disease of the inner ear, whose clinical symptoms include episodic vertigo, nausea, vomiting, fluctuating hearing loss, and tinnitus, and whose main change is the accumulation of water in the membranous labyrinth. The establishment of an animal model of membranous labyrinthine effusion may help to explore the effects of chronic membranous labyrinthine effusion on auditory function and the dehydrating effect of glycerol experiments.
Principle
The basic principle of the cochlear labyrinth modeling experiments is that in the animal experiments of surgical destruction of the endolymphatic capsule, it was found that balloon effusion and cochlear duct effusion occurred at about 10 days after the surgery, and that 30 days after the destruction of the endolymphatic capsule, the cochlear labyrinth was dilated so that the vestibular membrane was almost touching the roof of the vestibular order, and the cochlea thus took up the entire vestibular order, and at the same time, it could be seen that balloon effusion had caused the balloon membrane to be directly on top of the stapes bone. The balloon was also seen to be hydrated so that the membrane of the balloon was directly on the stapes base plate, but the ellipsoidal bursa and semicircular canals did not show any significant hydration.
Operation method
Modeling experiments in an animal model of membranous labyrinthine effusion
Principle
The basic principle of the cochlear labyrinth modeling experiments is that in the animal experiments of surgical destruction of the endolymphatic capsule, it was found that balloon effusion and cochlear duct effusion occurred at about 10 days after the surgery, and that 30 days after the destruction of the endolymphatic capsule, the cochlear labyrinth was dilated so that the vestibular membrane was almost touching the roof of the vestibular order, and the cochlea thus took up the entire vestibular order, and at the same time, it could be seen that balloon effusion had caused the balloon membrane to be directly on top of the stapes bone. The balloon was also seen to be hydrated so that the membrane of the balloon was directly on the stapes base plate, but the ellipsoidal bursa and semicircular canals did not show any significant hydration.
Materials and Instruments
Equipment: Move The basic process of modeling experiments in the animal model of membrane labyrinthine effusion can be divided into the following steps, with guinea pigs as the experimental animals: A A horizontal inferotemporal line was made along the foramen magnum of the occipital bone, and then a vertical imaginary line was made along the right occipital condyle, and a small hole of about 1 mm in diameter was drilled at the intersection of these two imaginary lines with an electric drill until the cancellous bone was removed, and the medial bone of the occipital bone was lifted up to the dense bony wall with a freewheeling forceps to expose the sigmoid sinus. B. The posterior border of the temporal bone at the junction of the temporal bone and the occipital bone can be seen at the lower part of the outer part of the operative field of the hole drilled from the occipital bone with a diameter of about 1 mm, and the sigmoid sinus can be seen in the inner part of the field of the hole from the upper part down to the inner part of the sigmoid sinus, and the temporal slit where the endolymphatic capsule is located is located in the crevice between the posterior border of the temporal bone and the sigmoid sinus. After exposing the endolymphatic capsule, the endolymphatic capsule can be fully disrupted by stabbing the endolymphatic capsule with a steel needle into the crevice where it is located. (Figures 9-13-18 and 9-13-19). For more product details, please visit Aladdin Scientific website.
Electric drill, hairspring tweezers, steel pins, etc.