Experiments on companion inheritance in Drosophila

Summary

Through this experiment to correctly recognize the difference between orthogamy and transgamy of companion inheritance, and to master the characteristics of companion inheritance. Source: Laboratory Course in Genetics

Operation method

hybridization

Principle

The red eye and white eye of Drosophila is a pair of relative traits controlled by genes on the sex chromosomes. With red-eyed female Drosophila and white-eyed male Drosophila, the F1 generation of both male and female red-eyed Drosophila, F1 generation of each other, F2 generation of females are red-eyed, males red-eyed: white-eyed = 1:1; on the contrary, with white-eyed females and red-eyed males Drosophila, F1 generation of females are red-eyed, males are white-eyed, F1 inter-mating to get the F2 generation, females red-eyed and white-eyed 1:1, males red-eyed and white-eyed ratio is 1:1. This shows that the genes located on the sex chromosomes are related to the sex of males and females.

Materials and Instruments

Drosophila wild type Drosophila realistica
Ether Cornstarch Agar Brown sugar Yeast powder Propionic acid.
Microscope Slides Coverslips

Move

1. Collection of virgin flies. The original adult flies of the cultured experimental material were poured out 8 h before hybridization. The female flies hatched after this are the virgin flies. If you do not do hybridization immediately after collection, you can separate the collected male and female Drosophila and culture them separately.

2. Make forward and backward crosses. The anesthetized parent fruit flies should be crossed according to the hybridization requirements, and each tube should be connected with 5 pairs of fruit flies, labeled, and incubated at 25℃.

3. Invert the parent. 7d later, when the F1 larvae appear, release the parent Drosophila (make sure to release it cleanly). 4.

4. After 3~4d, observe the traits of theF1 adults (pay attention to the difference between forward and backward crosses , and examine the relationship between eye color and sex). 5.

5. After anesthetizing the emerging F1 male and female Drosophila, select 5 pairs of Drosophila and put them into a new medium for further cultivation (no need to use virgin flies here). The F1 individuals of the two combinations should not be mixed and should be cultured separately. 6.

6. Release the clean F1 generation of parental Drosophila after 7d. 7.

7. After another 3~4d, the F2 generation of adult flies appeared, and after anesthesia, the eye color and sex were carefully observed on a white porcelain plate, and statistics were made. 8.

8. Count every 1~2d and accumulate the data for 6~7d.

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