The establishment of a high metastasis model of human gastric cancer can (1) study the mechanism of tumor metastasis; (2) study the prevention and treatment of tumors; and (3) provide a more ideal animal model for the study of tumor metastasis.
Operation method
In situ transplantation of tumor tissue blocks
Principle
When human gastric cancer tissue block SGC-7901 was transplanted in situ in SCID mice, the metastasis rate of the transplanted tumor to lymph nodes and liver at the end of the 4th week was 66.7%, and the metastasis rate at the end of the 6th week was as high as 100%.
Materials and Instruments
Male SPF grade SCID mice Human gastric cancer tissue SGC-7901 Move I. Preparation of experimental materials Caveat Tissue transfer should follow strict biosafety standards to avoid tissue contamination. Common Problems In situ transplantation includes two methods: in situ inoculation of human tumor cell lines, or in situ transplantation of human tumor tissue blocks to corresponding organs in nude mice. In situ inoculation of human cancer cell lines can increase the metastatic rate of a few tumors, such as colon cancer and pancreatic cancer, but cannot increase the metastatic rate of human tumors, such as gastric cancer, in nude mice. In situ transplantation of human gastric cancer tissue blocks was used to establish a gastric cancer metastasis model in nude mice, and the metastasis rate of the model established by this method was as high as 100% (metastasis to lymph nodes, liver, lungs and other organs). The in situ transplantation of cancerous tissue blocks to the corresponding organs in nude mice could better reflect the infiltration and metastatic characteristics of human tumors, probably due to the preservation of certain structures between cancer cells.SCID mice are severe combined immunodeficiency mice, with lower immune function than nude mice, and therefore are more suitable for metastasis models. For more product details, please visit Aladdin Scientific website.
Chloral hydrate Formalin solution
Breeding cage Optical microscope Paraffin wax
1. male SPF grade SCID mice, 6-7 weeks old, weighing 20-25 g. 2. subcutaneous human gastric cancer tissue (SGC-7901, low-differentiated adenocarcinoma).
2. subcutaneous human gastric cancer tissue (SGC-7901, poorly differentiated adenocarcinoma) from nude mice.
Establishment of high metastasis model of gastric cancer
1. 25 SCID mice were randomly divided into test group (n=15) and normal control group (n=10). 2.
2. In situ transplantation of human gastric cancer tissue SGC-7901 in the experimental group: after anesthetizing the animals with 4.3% chloral hydrate, the skin, abdominal wall and peritoneum of the left epigastric region were incised sequentially to carefully expose the gastric wall, and the gastric plasma membrane was damaged with scissors in the middle of the greater curvature of the stomach (about 3 mm in diameter), and a block of human gastric cancer tissue (about 5 mmx7 mm, weighing 150 mg) was sewn onto the damaged area with a silk thread. Then the stomach was sent into the abdominal cavity, and the abdominal wall and skin were closed sequentially with No. 0 suture. 3.
3. After the operation, the animals were observed under anesthesia until all of them were awake, and then they were sent to the feeding room. 4.
4. The normal control group was not treated.
5. 2, 3 and 2 animals of the test group were randomly executed at the end of the 2nd, 4th and 6th weeks after surgery, respectively. It was found that the cancer had metastasized in 2 animals at the end of the 6th week, so the remaining 8 SCID mice were also executed at the end of the 6th week.
6. The locally grown tumor tissues in the stomach, enlarged lymph nodes, brain, and organs in the thoracic and abdominal cavities were taken, fixed in 10% formalin solution, embedded in paraffin wax, routinely prepared, stained with HE staining, and the growth and metastasis of the tumors were observed and recorded under a light microscope.