LC-MS analysis can be applied to (1) the detection of toxic substances in the body and (2) pharmaceutical research.
Operation method
LC-MS method
Principle
The operating instructions accompanying the LC-MS provide a precise and sensitive method for the analysis of drugs and toxic substances in GUS.Advantages of the LC-MS GUS analytical method: Sample preparation is included, new analytes can be easily added to the screening method, and there are no limitations on the volatility or thermal stability of the substance. In addition, the user-friendly software design (ToxIDTM 1.0) eliminates the need to manually analyze each sample chromatogram and automatically generates concise and detailed result report forms.
Materials and Instruments
Urine Sample Move 1. Direct injection to obtain secondary mass spectrum and add to database 10 minutes 2. Sample on column to obtain retention time 13 or 30 minutes (based on method selection) 3. Update the parent ion table and standard retention time in Instrumental Methods 2 min. 4. Update name, parent ion, highest intensity secondary ion and retention time in ToxID software 2 minutes Caveat The well-established GUS methods described in the instructions are accomplished using any of the LXQ, LCQ Fleet ion traps containing the SPE program, of which there are two LC methods capable of identifying more than 275 compounds in human urine.The 30-minute version of this LC method is recommended to perform a thorough and comprehensive study of the analytes present in the sample. The 13-minute LC method is then recommended if the laboratory is required to obtain feedback in a short period of time. Also, since at the same time the ToxID software allows automated data analysis and reporting results. Thus, the need for manual data analysis is eliminated and confidence in compound identification is increased. Common Problems The 13-minute and 30-minute LC-MS-based methods allow for the identification of more analytes than other routine unknown screening methods, and one of the most important features of the GUS workflow is the ability to easily add new analytes to the screening method. This aspect of the application software is also important for routine unknown screening, so that new analytes can be added to the target list on an ongoing basis. For more product details, please visit Aladdin Scientific website.
Ion Trap Mass Spectrometer HPLC Columns