β-Glucosidase, CAS No.9001-22-3

CAS: 9001-22-3 Cat. No.: G755204 EC Number: 232-589-7

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥4 U/mg powder

Bioactivity

≥4 U/mg powder

Overview

Catalyzes the hydrolysis of glucosides. Emulsin originally was the term applied to the extract of sweet almonds. It remains as the historical prototype of β-glucosidase. Many other sources of the enzyme have been described. Emulsin also shows significant D-galactosidase activity and there is some question as to whether or not it is one enzyme with two activities, because non-parallel response to inhibitors points to two separate enzymes. D-mannosidase has also been reported in almond emulsin.

Form: Supplied as a light yellow to yellow tan powder.
Solubility: Colorless to light yellow, clear to slightly hazy in water.
Solubility Concentration:1.90-2.10mg/ml

Assay Method: Salicin is hydrolyzed by b-glucosidase to yield saligenen and b-D-glucose. The rate of formation of glucose is measured in a hexokinase/glucose-6-phosphate dehydrogenase system. One unit releases one micromole of glucose per minute at 37°C and pH 5.0 under the specified conditions.
Specificity: b-glucosides and b-galactosides are reportedly substrates for the enzyme.
Inhibitors: HgCl₂, other heavy metal ions, sulfhydryl-binding compounds and polyols.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.

Specifications

Product Name

β-Glucosidase, CAS No.9001-22-3

Synonyms

beta-glucosidase | amygdalase | beta-D-glucoside glucohydrolase | cellobiase | gentobiase | β-D-Glucoside glucohydrolase | b-D-Glucosidase | b-D-Glucoside glycohydrolase

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥4 U/mg powder

Biochemical and Physiological Mechanisms

βglucosidase is involved in the hydrolysis of β-glycosidic bonds connecting carbohydrate residues in β-D-glycosides. They convert cellobiose and cellooligosaccharides produced by the endo and exoglucanases to glucose.

Bioactivity

≥4 U/mg powder

Predicted molecular weight

~135000

CAS

9001-22-3

Enzyme Commission Number

EC 3.2.1.21

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥4 U/mg powder

Reconstitution

Reconstitute with sterile ddH2O. Store at 4°C.

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at -8℃ long term (1 year). Upon reconstitution, it is recommended to aliquot.

Unit definition

1 unit releases 1umole of glucose per minute from salicin at 37ºC, pH 5.0.

References

1. Shi Cong-Rong, Zhang Xiao-Juan, Xie Han, Zhang Wei, Tang Rui-Xin, Lu Zhen-Ming, Geng Yan, Lu Ran, Wang Fang-Zhou, Chai Li-Juan, Zhang Hui-Ling, Xu Hong-Yu, Shi Jin-Song, Xu Zheng-Hong.  (2026)  Metabolomic profiling of goji fermentation: enhancing functional metabolites with high-glucose-adaptive LAB.  npj Science of Food,      [PMID:] [10.1038/s41538-026-00791-x]