IL-6 Antibody - Primary antibody, specific to IL-6

Cat. No.: Ab110374

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human IL-6 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

IL-6 Antibody - Primary antibody, specific to IL-6

Synonyms

Interleukin BSF 2 antibody | B cell differentiation factor antibody | B cell stimulatory factor 2 antibody | B-cell stimulatory factor 2 antibody | BSF 2 antibody | BSF-2 antibody | BSF2 antibody | CDF antibody | CTL differentiation factor antibody | Cyto

Specifications & Purity

ExactAb™, Validated, 1.0 mg/mL

Biochemical and Physiological Mechanisms

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induc

Host species

Rabbit

Specificity

IL-6

Immunogen

A synthetic peptide derived from human IL-6 (AA 131-180)

Positive Control

WB: K562 lysates. IHC: Human lung tissue. IF/ICC: A549 cells.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

SDS-PAGE

150 kDa

Purification Method

Immunogen affinity purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: IL-6 Antibody (Ab110374)

IL-6 Antibody (Ab110374) - Western Blot
All lanes: IL-6 Antibody (Ab110374) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution
Predicted band size: 23 kDa
Observed band size: 19 kDa
Exposure time: 80.0 s

IHC: IL-6 Antibody (Ab110374)

IL-6 Antibody (Ab110374) - IHC
Immunohistochemical analysis of paraffin-embedded human-lung, IL-6 Antibody (Ab110374) was diluted at 1/100

IF/ICC: IL-6 Antibody (Ab110374)

IL-6 Antibody (Ab110374) - IF/ICC
Immunofluorescence analysis of A549. IL-6 Antibody (Ab110374) (red) was diluted at 1/200 (4°C, overnight). Goat Anti Rabbit IgG (H&L) - AF 594
Secondary antibody was diluted at 1/1000 (room temperature, 50 min). Picture B: DAPI(blue) 10 min.

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/100-1/300

• IF/ICC:

  1/100-1/300;

Associated Targets(Human)

IL6 Tclin Interleukin-6 (0 Activities)

Discover Target: IL6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

References

1. Subramani Kandhasamy, Baofang Wu, Jiayin Wang, Xiaojing Zhang, Gao Hongzhi, Da-Peng Yang, Yiming Zeng.  (2024)  Tracheal regeneration and mesenchymal stem cell augmenting potential of natural polyphenol-loaded gelatinmethacryloyl bioadhesive.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:38772466] [10.1016/j.ijbiomac.2024.132506]