NKG2D/CD314 Rat mAb (AF488) - Primary antibody, specific to NKG2D/CD314

Cat. No.: Ab211176

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG1

Clone Number

CX5

Conjugation

AF488

Overview

at anti Mouse NKG2D/CD314 Antibody, Monoclonal (CX5), could be used for Flow Cytometry.

Specifications

Product Name

NKG2D/CD314 Rat mAb (AF488) - Primary antibody, specific to NKG2D/CD314

Synonyms

Nkg2d | D6H12S2489E | CD314 antigen | CD314 | D12S2489E | FLJ17759 | FLJ75772 | Killer cell lectin-like receptor subfamily K member 1 | killer cell lectin-like receptor subfamily K, member 1 | KLR | KLRK1 | NK cell receptor D | NKG2-D type II integral mem

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymph

Host species

Rat

Specificity

NKG2D/CD314

Immunogen

Purified mouse NKG2D

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

AF488

Excitation (nm)

Blue Laser(488nm)

Emission(nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: NKG2D/CD314 Rat mAb (AF488) (Ab211176)

NKG2D/CD314 Rat mAb (AF488) (Ab211176) - Flow Cytometry
Two-color flow cytometric analysis of NKG2D/CD314 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes cells were stained with NKG2A/C/E Rat mAb (AF647) (Ab212165) and either Rat IgG (AF488) (Ab190240; Left panel) or NKG2D/CD314 Rat mAb (AF488) (Ab211176; Right panel) at 0.2μg/test.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test. For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

KLRK1 Tbio NKG2-D type II integral membrane protein (0 Activities)

Discover Target: KLRK1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)