Recombinant Endo H (MS Grade), CAS No.37278-88-9

CAS: 37278-88-9 Cat. No.: rp227292 EC Number: 998-699-9

GRADE & PURITY Animal Free, Carrier Free, Bioactive, Recombinant, Suitable for mass spectrometry (MS), ActiBioPure™, EnzymoPure™, for Protein sequencing, expressed in E.coli

Expression system

E. coli

Overview

  Endo H (Endoglycosidase H), also known as endoglycosidase H, is cloned from Streptomyces plicatus and recombinantly expressed in E. coli. Endo H cleaves within the chitobiose core of high-mannose and some hybrid oligosaccharides from N-linked glycoproteins. It hydrolyzes the N-linked glycan chain between two N-acetylglucosamine residues, resulting in a glycoprotein structure with a single N-acetylglucosamine remaining attached. Endo H is used to determine protein glycosylation status, characterize glycan structures, and remove protein glycosylation.

Components

rp227292	Component	10KU	Storage
rp227292A	Endo H (10 KU/mL)	10 KU	-20°C. Avoid freeze/thaw cycles.
rp227292B	10× Reaction Buffer (500 mM Sodium Acetate)	2 mL	-20°C. Avoid freeze/thaw cycles.
rp227292C	10× Denaturation Buffer (5% SDS, 400 mM DTT)	1 mL	-20°C. Avoid freeze/thaw cycles.

Product Features

High Purity: No contaminating proteases or other glycosidases; purity ≥ 95%.

Consistent Quality: Strict quality control with activity and functional verification for each batch to ensure performance.

Broad Compatibility: Glycerol-free formulation; the digestion system is compatible with HPLC and mass spectrometry workflows.

Protocol

1. Reagent Preparation

Dissolve the Endo H lyophilized powder in 1 mL ddH₂O to prepare a 10,000 U/mL working solution. It is recommended to aliquot the solution according to single-use volumes and store at -20°C to minimize contamination.

2. Reaction Setup

Step 1: Denaturation Reaction

Component	Volume
10× Denaturation Buffer (5% SDS, 400 mM DTT)	1 μL
Substrate Protein	1–20 μg
ddH₂O	Add to 10 μL

Reaction conditions: 95°C for 5 min, then cool to room temperature.

Step 2: Digestion Reaction 

Component	Volume
10× Reaction Buffer (500 mM Sodium Acetate)	2 μL
Denatured sample from Step 1	10 μL
Endo H	1–5 μL
ddH₂O	Add to 20 μL

Reaction conditions: Incubate at 37°C for 1 h.

Storage Conditions

Lyophilized: Stable for 1 year when stored properly.

After reconstitution: Stable for 1 year at -20°C, provided repeated freeze/thaw cycles are avoided.

Precautions

1. For research use only. Not for use in clinical diagnosis or treatment, or in food or pharmaceutical products.
2. Wear appropriate personal protective equipment, including a lab coat, disposable gloves, and a face mask, when handling this product.
3. Avoid repeated freeze/thaw cycles.

Specifications

Product Name

Recombinant Endo H (MS Grade), CAS No.37278-88-9

Synonyms

Endo H

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, for Protein sequencing, Recombinant, Suitable for mass spectrometry (MS), EnzymoPure™

Specifications & Purity

Animal Free, Carrier Free, Bioactive, Recombinant, suitable for mass spectrometry (MS), ActiBioPure™, EnzymoPure™, for protein sequencing, expressed in E.coli

Predicted molecular weight

77.38KD

SDS-PAGE

77.38KD

CAS

37278-88-9

Enzyme Commission Number

3.2.1.96

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in E.coli

Reconstitution

Dissolve the lyophilized powder with 1 ml of ddH2O to prepare an enzyme working solution of 10,000 U/ml.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (12months).

Unit definition

The amount of enzyme required to release more than 95% of the carbohydrates from 10 μg of denatured ribonuclease B in a 10 μl reaction system within 1 hour at 37℃ is defined as one enzyme activity unit.