Determine the necessary mass, volume, or concentration for preparing a solution.
Recombinant FGF2 Antibody (Ab103298) - Western Blot
All lanes: Recombinant FGF2 Antibody (Ab103298) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 17, 22, 25 kDa. Recombinant FGF2 Antibody (Ab103298) recognizes three isoforms of FGF2. The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).
Exposure time: 6.3 s

