RPL10A Antibody - Primary antibody, specific to RPL10A, Rabbit IgG

Cat. No.: Ab125777
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. 1.0 mg/mL
Application
IF/ICC, IHC, WB
Host species
Rabbit
Reactive species
Human, Mouse, Rat
Isotype
Rabbit IgG
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab125777-10μl
1
$99.90
50μl
Ab125777-50μl
1
$269.90
100μl
Ab125777-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab125777-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$2,799.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Validated, 1.0 mg/mL Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rabbit anti Human RPL10A Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 1/500-1/2000
IHC: 1/50 - 1/300

IF/ICC: 1/200

Specifications

Product Name
RPL10A Antibody - Primary antibody, specific to RPL10A, Rabbit IgG
Synonyms
60S ribosomal protein L10a | CSA 19 | CSA-19 | CSA19 | L10A | NEDD 6 | NEDD-6 | NEDD6 | neural precursor cell expressed developmentally down regulated protein 6 | Neural precursor cell expressed developmentally down-regulated protein 6 | Protein NEDD6 | R
Specifications & Purity
Validated, 1.0 mg/mL
Host species
Rabbit
Specificity
RPL10A
Immunogen
A synthetic peptide derived from human RPL10A (AA 30-110).
Positive Control
WB: HeLa, Hep G2, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

RPL10A Antibody (Ab125777) - Western Blot
All lanes: RPL10A Antibody (Ab125777) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/10000 dilution

Predicted band size: 25 kDa
Observed band size: 26 kDa
Exposure time: 22.0 s

RPL10A Antibody (Ab125777) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using RPL10A Antibody (Ab125777). Antibody was diluted at 1/200 (4° overnight). Tris-EDTA, pH9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45min).

RPL10A Antibody (Ab125777) - IF/ICC
IF analysis of RPL10A Antibody (Ab125777) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with RPL10A Antibody (Ab125777) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application
ApplicationDilution info
WB

1/500-1/2000

IF/ICC

1/200

IHC

1/50-1/300

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
RPL10A Tbio 60S ribosomal protein L10a (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F1012348Certificate of AnalysisMay 20, 2026 Ab125777
ZJ24F1012349Certificate of AnalysisMay 20, 2026 Ab125777
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

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