Recombinant Human Hsp60 Protein, >95% (SDS-PAGE)

Cat. No.: rp169616

GRADE & PURITY Carrier Free, Azide Free, His Tag, ≥95%(SDS-PAGE)

Expression System

E. coli

Accession #

P10809

Protein Tag

N-His

Expression system

E. coli

Overview

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
HSPD1, also known as HSP60, is a member of the chaperonin family. HSPD1 may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. It may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. HSPD1 gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13). Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4); also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. HSPD1 is clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurs within the first two decades of life.

Specifications

Product Name

Recombinant Human Hsp60 Protein, >95% (SDS-PAGE)

Synonyms

60 kDa chaperonin | Chaperonin 60 | CPN60 | EC 5.6.1.7 | Heat shock protein 60 | Hsp60 | HSP-60 | HuCHA60 | Mitochondrial matrix protein P1 | P60 lymphocyte protein | 60 kDa chaperonin | Chaperonin 60 (CPN60) | Heat shock protein 60 (HSP-60 | Hsp60) | HuC

Grade

Azide Free, Carrier Free, His Tag

Specifications & Purity

Carrier Free, Azide Free, His-Tag, ≥95%(SDS-PAGE)

Purity

>95% (SDS-PAGE)

Expression System

E. coli

Amino Acids

1-573 aa

Sequence

MGSSHHHHHHSSGLVPRGSHMLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF

Protein Tag

N-His

Accession #

P10809

Predicted molecular weight

63.2 kDa

SDS-PAGE

62.0 kDa, under reducing conditions.

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year, store at 2-8℃ for 1 month. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human Hsp60 Protein (rp169616)

Recombinant Human Hsp60 Protein (rp169616) - SDS-PAGE
3 μg/lane of Recombinant Human Hsp60 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 62.0 kDa under reducing conditions.