Recombinant Human p53 Protein, >90% (SDS-PAGE)

Cat. No.: rp170134

GRADE & PURITY Carrier Free, Azide Free, His Tag, ≥90%(SDS-PAGE)

Expression System

E. coli

Accession #

P04637

Protein Tag

N-His

Expression system

E. coli

Overview

Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis, including cell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.

Specifications

Product Name

Recombinant Human p53 Protein, >90% (SDS-PAGE)

Synonyms

Antigen NY-CO-13 | BCC7 | Cellular tumor antigen p53 | FLJ92943 | LFS1 | Mutant tumor protein 53 | p53 | p53 tumor suppressor | P53_HUMAN | Phosphoprotein p53 | Tp53 | Transformation related protein 53 | TRP53 | tumor antigen p53 | Tumor protein 53 | Tumo

Grade

Azide Free, Carrier Free, His Tag

Specifications & Purity

Carrier Free, Azide Free, His-Tag, ≥90%(SDS-PAGE)

Purity

>90% (SDS-PAGE)

Expression System

E. coli

Amino Acids

1-393 aa

Sequence

MGSSHHHHHHSSGLVPRGSHMEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD

Protein Tag

N-His

Accession #

P04637

Predicted molecular weight

45.8 kDa

SDS-PAGE

55.4 kDa, under reducing conditions; 52.5 and 122.4 kDa, under non-reducing conditions

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human p53 Protein (rp170134)

Recombinant Human p53 Protein (rp170134) - SDS-PAGE
2 μg/lane of Recombinant Human p53 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 55.4 kDa under reducing conditions and 52.5 & 122.4 kDa under non-reducing conditions.