GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.2.1 mg/mL
ExactAb™, Validated, Recombinant, 2.1 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human Nck-2 Antibody, Recombinant, could be used for WB, IF, ICC and so on.
Application: WB: 1/500-1/1000 IF/ICC: 1/50-1/200
Protein Function Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
Specifications
Product Name
Recombinant Nck-2 Antibody - Primary antibody, specific to NCK2, Rabbit IgG
Synonyms
Cytoplasmic protein NCK2 | NCK2 | GRB4 | Growth factor receptor bound protein 4 | NCK adaptor protein 2 | Nck-2 | NCK2_HUMAN | NCKbeta | Non-catalytic region of tyrosine kinase adaptor protein 2 | SH2/SH3 adaptor protein NCK-beta | Noncatalytic region of
Specifications & Purity
ExactAb™, Validated, Recombinant, 2.1 mg/mL
Host species
Rabbit
Specificity
NCK2
Immunogen
A synthetic peptide derived from human Nck beta (AA 75-88)
Positive Control
WB: K562, SK-N-SH and HEK293 cell lysates. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
2.1 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Nck-2 Antibody (Ab117240) - Western Blot All lanes: Recombinant Nck-2 Antibody (Ab117240) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 45 kDa Exposure time: 126.7 s
Recombinant Nck-2 Antibody (Ab117240) - IF/ICC Immunocytochemistry analysis of Nck beta (green) in HeLa using Recombinant Nck-2 Antibody (Ab117240) and DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IF/ICC
1/50 - 1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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